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Browsing by Author "Brook, Ryan K."

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    A comparison of wild boar and domestic pig microbiota does not reveal a loss of microbial species but an increase in alpha diversity and opportunistic genera in domestic pigs
    (American Society for Microbiology, 2024-08-20) Rahman, Rajibur; Fouhse, Janelle; Ju, Tingting; Fan, Yi; Schultz Marcolla, Camila; Pieper, Robert; Brook, Ryan K.; Willing, Benjamin Peter
    The microbiome of wild animals is believed to be co-evolved with host species, which may play an important role in host physiology. It has been hypothesized that the rigorous hygienic practices in combination with antibiotics and diets with simplified formulas used in the modern swine industry may negatively affect the establishment and development of the gut microbiome. In this study, we evaluated the fecal microbiome of 90 domestic pigs sampled from nine farms in Canada and 39 wild pigs sampled from three different locations on two continents (North America and Europe) using 16S rRNA gene amplicon sequencing. Surprisingly, the gut microbiome in domestic pigs exhibited higher alpha-diversity indices than wild pigs (P < 0.0001). The wild pig microbiome showed a lower Firmicutes-to-Bacteroidetes ratio and a higher presence of bacterial phyla Elusimicrobiota, Verrucomicrobiota, Cyanobacteria, and Fibrobacterota when compared to their domestic counterparts. At the genus level, the wild pig microbiome had enriched genera that were known for fiber degradation and short-chain fatty acid production. Interestingly, the phylum Fusobacteriota was only observed in domestic pigs. We identified 31 ASVs that were commonly found in the pig gut microbiome, regardless of host sources, which could be recognized as members of the core gut microbiome. Interestingly, we found five ASVs missing in domestic pigs that were prevalent in wild ones, whereas domestic pigs harbored 59 ASVs that were completely absent in wild pigs. The present study sheds light on the impact of domestication on the pig gut microbiome, including the gain of new genera, which might provide the basis to identify novel targets to manipulate the pig gut microbiome for improved health.
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    Temporal aspects of polar bear (Ursus maritimus) occurrences at field camps in Wapusk National Park, Canada
    (Springer Berlin Heidelberg, 2017-08) Laforge, Michel P.; Clark, Douglas A.; Schmidt, Aimee L.; Lankshear, Jessica L.; Kowalchuk, Sheldon; Brook, Ryan K.
    Interaction between polar bears (Ursus maritimus) and people is a growing concern for both bear conservation and human safety in a warming Arctic climate. Consequently, the importance of monitoring temporal trends in the proximity of polar bears to people has become critical in managing human-polar bear conflicts. Such concerns are acute in Wapusk National Park in Manitoba, Canada on the Western Hudson Bay coast, where we deployed 18 camera traps at three remote field camps from 2010–2014 (~22,100 camera-days) to monitor the frequency and timing of bears’ visits to those facilities. Following seasonal breakup of Hudson Bay’s sea ice polar bear occurrences at these camps increased throughout the summer and into fall (low in May–July and increasing sharply through August–November and then approaching zero in December when Hudson Bay freezes). We quantified age and sex class and estimated body condition of bears visiting the camps: adult males were most prevalent at Nester One camp close to where adult males congregate at Cape Churchill, whereas the two camps farther south were visited more frequently by females with dependent young, likely traveling to and from a known maternal denning area. Few subadults were observed. As expected, body condition scores declined throughout the on-shore season. Our method of monitoring polar bear occurrence on shore is robust, cost-effective, and non-invasive, and so may provide an economical complement to data gathered through more conventional techniques.