Browsing by Subject "yeast two-hybrid assay"
Now showing items 1-4 of 4
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Analysis of functional domains required for hRad18 interactions with HHR6B and hUbc9
(2006-10-26)DNA post-replication repair (PRR) is a cellular tolerance mechanism by which eukaryotic cells survive lethal lesions during or after DNA synthesis. In the yeast Saccharomyces cerevisiae, modification of proliferating cell ... -
Characterization of the Ubc13-Mms2 Lysine-63-linked ubiquitin conjugating complex
(2006-04-21)Ubiquitylation is an indispensable post-translational modification system in eukaryotic cells that leads to the covalent attachment of a small ubiquitin (Ub) protein onto a target. The traditional and best-characterized ... -
Identification of protein-protein interactions in the type two secretion system of aeromonas hydrophila
(2009)The type II secretion system is used by many pathogenic and non-pathogenic bacteria for the extracellular secretion of enzymes and toxins. Aeromonas hydrophila is a Gram-negative pathogen that secretes proteins via the ... -
Structural and functional characterization of the budding yeast Mus81-Mms4 complex
(2003-06-27)The Saccharomyces cerevisiae Mms4 and Mus81 proteins are required for repairing DNA alkylation damage, but not damage caused by ionizing radiations. Previous studies have demonstrated that Mms4 and Mus81 form a specific ...