Temperature, pH, and media requirements for bacterial antagonism of soilborne pathogens
Peer Reviewed StatusNon-Peer Reviewed
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Select rhizobacteria, Pseudomona fluorescens strain 63-49 and P. cepacia strain Ral-3, were evaluated for their ability to suppress the growth of several isolates of Rhizoctonia solani and Cochliobolus sativus, respectively, in liquid medium, on several solid media, at different temperature regimes and at varying pH. The two bacterial strains tested significantly reduced the growth of the fungi, irrespective of the growth medium, temperature or pH. Among the solid media tested, the efficacy of bacteria against fungi was best on Potato Dextrose Agar (PDA) for strain 63-49 and Pseudomonas Agar F (PAF) for strain Ral-3. However, liquid medium results were quite variable depending on the fungal isolate especially for C. sativus. The activity of strain 63-49 was highest at 22°C and the activity of strain Ral-3 was at 30°C.
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