University of SaskatchewanHARVEST
  • Login
  • Submit Your Work
  • About
    • About HARVEST
    • Guidelines
    • Browse
      • All of HARVEST
      • Communities & Collections
      • By Issue Date
      • Authors
      • Titles
      • Subjects
      • This Collection
      • By Issue Date
      • Authors
      • Titles
      • Subjects
    • My Account
      • Login
      JavaScript is disabled for your browser. Some features of this site may not work without it.
      View Item 
      • HARVEST
      • Electronic Theses and Dissertations
      • Graduate Theses and Dissertations
      • View Item
      • HARVEST
      • Electronic Theses and Dissertations
      • Graduate Theses and Dissertations
      • View Item

      A Nutrigenomic Perspective to Search for Gene Variants That Influence Carcass Traits of Feedlot Cattle

      Thumbnail
      View/Open
      HAMILTON-THESIS-2019.pdf (1.422Mb)
      Date
      2019-01-17
      Author
      Hamilton, Kelsey Evelyn 1994-
      ORCID
      0000-0002-5178-5739
      Type
      Thesis
      Degree Level
      Masters
      Metadata
      Show full item record
      Abstract
      Vitamin A (VA) has a nutrigenomic effect on intramuscular fat. Discovering variants in genes involved in fat deposition that are also affected by vitamin A could allow feedlots to precision feed to optimize carcass traits. A single nucleotide polymorphism (SNP) in alcohol dehydrogenase 1C (ADH1C) has previously shown promise for this approach but has yet to be effective at a commercial level; therefore we hypothesized a variant in another gene or its interaction with ADH1Cc.-64T>C might be the solution. Genes previously shown to be affected by retinoic acid, a metabolite of vitamin A: aminopeptidase (ANPEP), clusterin (CLU), adipose differentiation-related protein (ADFP), glutathione peroxidase (GPX3), secreted protein, acidic, cysteine-rich (SPARC), and insulin growth factor binding protein 6 (IGFBP6) were sequenced and screened for variants. The ANPEPc.410G>A SNP was selected for genotyping in a population of mixed breed steers (n=988). This population was fed vitamin A at 100% (100VA) or 50% (50VA the NRC recommended level (2200 IU/kg dry matter). No interaction was found with ADH1Cc.-64T>C however, ANPEPc.410G>A affected carcass yield (P<0.01; AA=2.47±0.03, GA=2.36±0.03, GG=2.14±0.08), marbling score (P<0.01; AA=397.2±2.7, GA=388.6±3.3, GG=370.4±7.2), and fat (P<0.01; AA=8.52±0.17 GA=7.58±0.21, GG=7.04±0.44; mm). Vitamin A also had an effect on backfat (P<0.05; 100VA= 8.13±0.24, 50VA = 7.35±0.25), and an interaction with ANPEPc.410c.G>A affected rib-eye area (P<0.05). The ANPEP SNP was genotyped in a second population of mixed breed steers (N=708) fed a standard feedlot ration with the NRC recommended level of vitamin A. There was an association with yield, marbling, fat, and rib-eye area (P<0.01). The AA genotype was more marbled, while GG animals were leaner with higher yields. Interestingly, ANPEPc.410G>A is the fourth variant in a haplotype containing twelve SNPs that are in linkage disequilibrium in exon 1 and intron 1. This was confirmed by sequencing cattle of various breeds from different populations. The three haplotypes could affect gene expression by altering transcription or translation efficiency. Investigation of the functional effects of these variants needs to be completed in order to understand how it alters traits related to feedlot cattle performance.
      Degree
      Master of Science (M.Sc.)
      Department
      Animal and Poultry Science
      Program
      Animal Science
      Supervisor
      Buchanan, Fiona C
      Committee
      Asai-Coakwell, Mika; McKinnon, John J; Bett, Kirstin E; Brook, Ryan K
      Copyright Date
      June 2019
      URI
      http://hdl.handle.net/10388/11739
      Subject
      beef
      cattle
      genetics
      variants
      marbling
      feedlot
      carcass
      nutrigenomics
      Collections
      • Graduate Theses and Dissertations
      University of Saskatchewan

      University Library

      The University of Saskatchewan's main campus is situated on Treaty 6 Territory and the Homeland of the Métis.

      © University of Saskatchewan
      Contact Us | Disclaimer | Privacy