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      • HARVEST
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      Initial Characterisation of a Novel Role of Shugoshin in Ciliated Neurons of Caenorhabditis elegans

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      WADDELL-THESIS-2019.pdf (6.597Mb)
      Date
      2019-07-17
      Author
      Waddell, Brandon 1993-
      Type
      Thesis
      Degree Level
      Masters
      Metadata
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      Abstract
      Across eukaryotic species, Shugoshin proteins perform several critical functions in meiotic and mitotic cells that ensures faithful chromosome segregation and the preservation of genomic stability. In the centromere, they function as adaptor proteins, mediating spindle attachment and cohesin phosphorylation to promote sister chromatid association and delay anaphase entry. In centrosomes, Shugoshin maintains centriole cohesion and regulates centrosome maturation in preparation for spindle nucleation. These functions implicate Shugoshin in regulating transient microtubule-related structures in the cell. Here I introduce a new function of Shugoshin in yet another tubulin-derived structure, the cilium. Using Caenorhabditis elegans (C. elegans) as a model, I investigated the possible localization of SGO-1 in sensory cilia of adult neurons and in the embryonic primordia of sensory organs. Finally, I identified TAC-1, a member of a conserved microtubule regulator protein family, as an SGO-1 interacting protein. Together, these results suggest the involvement of a similar genetic toolkit in the regulation of diverse cellular functions and reveal the first evidence of Shugoshin activity in a fully differentiated cell type.
      Degree
      Master of Science (M.Sc.)
      Department
      Biology
      Program
      Biology
      Supervisor
      Carvalho, Carlos
      Committee
      Ambrose, Chris; Todd, Chris; Eskiw, Chris; Benson, James
      Copyright Date
      November 2019
      URI
      http://hdl.handle.net/10388/12196
      Subject
      Shugoshin
      C. elegans
      cilia
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