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      • HARVEST
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      Analysis of the Outcrossing Ratio of German Chamomile (Matricaria recutita L.) using SSR-markers

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      WOYTOWICH-THESIS-2020.pdf (3.371Mb)
      Date
      2020-05-26
      Author
      Woytowich, Maureen
      Type
      Thesis
      Degree Level
      Masters
      Metadata
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      Abstract
      German chamomile (Matricaria recutita L.) is one of the most ancient and widely used medicinal plant species. Plant breeding efforts for desired agronomic traits have existed for over sixty years; however, the breeding programs for chamomile are small in comparison to other crop plant species. The objective of this thesis was to examine the variability of chamomile’s outcrossing ratio based upon multiple environmental factors in order to provide a basic understanding of pollination biology to inform the breeding of chamomile. Two studies were conducted to achieve this objective. The first study evaluated the outcrossing ratio at multiple geographical locations throughout central Germany. The second study evaluated greenhouse compared to field conditions, as well as, potential temperature effects on the outcrossing ratio. Chamomile exhibited a wide range of outcrossing frequencies in the crosses regardless of both location and ploidy. The results did not show a statistical difference between geographical locations; however, in all crosses there appeared to be an effect associated with crossing direction. In many crosses, the frequency of outcrossed progeny was considerably higher in one cross direction than the other. The greenhouse and field comparison could not be made due to progeny germination difficulties. The greenhouse crosses could have been negatively affected by poor pollination, seed dormancy, and unfavorable temperature conditions during germination. Although this study had technical issues, and the outcrossing ratio could not be analyzed as expected, valuable knowledge was gained for the direction of future research in chamomile.
      Degree
      Master of Science (M.Sc.)
      Department
      Plant Sciences
      Program
      Plant Sciences
      Supervisor
      Sharbel, Tim
      Committee
      Chibbar, Ravi; Beattie, Aaron; Booker, Helen; Davis, Art
      Copyright Date
      April 2020
      URI
      http://hdl.handle.net/10388/12859
      Subject
      German chamomile
      Matricaria recutita
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      • Graduate Theses and Dissertations
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