Fast Quantification Without Conventional Chromatography, The Growing Power of Mass Spectrometry
Date
2020-06-08
Authors
gachumi, george
Purves, Randy
Hopf, Carston
El-Aneed, Anas
Journal Title
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Publisher
ACS Publications
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Article
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Abstract
Mass spectrometry (MS) in hyphenated techniques is widely accepted as the gold standard quantitative tool in life sciences. However, MS possesses intrinsic analytical capabilities that allow it to be a stand-alone quantitative technique, particularly with current technological advancements. MS has a great potential for simplifying quantitative analysis without the need for tedious chromatographic separation. Its selectivity relies on multistage MS analysis (MSn), including tandem mass spectrometry (MS/MS), as well as the ever-growing advancements of high-resolution MS instruments. This perspective describes various analytical platforms that utilize MS as a stand-alone quantitative technique, namely, flow injection analysis (FIA), matrix assisted laser desorption ionization (MALDI), including MALDI-MS imaging and ion mobility, particularly high-field asymmetric waveform ion mobility spectrometry (FAIMS). When MS alone is not capable of providing reliable quantitative data, instead of conventional liquid chromatography (LC)-MS, the use of a guard column (i.e., fast chromatography) may be sufficient for quantification. Although the omission of chromatographic separation simplifies the analytical process, extra procedures may be needed during sample preparation and clean-up to address the issue of matrix effects. The discussion of this manuscript focuses on key parameters underlying the uniqueness of each technique for its application in quantitative analysis without the need for a chromatographic separation. In addition, the potential for each analytical strategy and its challenges are discussed as well as improvements needed to render them as mainstream quantitative analytical tools. Overcoming the hurdles for fully validating a quantitative method will allow MS alone to eventually become an indispensable quantitative tool for clinical and toxicological studies.
Description
Keywords
Chromatography, Ionization, Ions, Mass spectrometry, Quantitative analysis
Citation
Gachumi G., Purves R.W., Hopf C., and El-Aneed A. (2020). Fast Quantification Without Conventional Chromatography, the Growing Power of Mass Spectrometry. Anal. Chem. 2020, 92, 13, 8628–8637. 10.1021/acs.analchem.0c00877
Degree
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Citation
Gachumi G., Purves R.W., Hopf C., and El-Aneed A. (2020). Fast Quantification Without Conventional Chromatography, the Growing Power of Mass Spectrometry. Anal. Chem. 2020, 92, 13, 8628–8637. 10.1021/acs.analchem.0c00877
Part Of
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DOI
10.1021/acs.analchem.0c00877
item.page.identifier.pmid
32510944