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      RAGE-DEPENDENT SENSITIZATION OF THORACIC SENSORY NEURONS DURING INFLAMMATORY CONDITIONS

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      GBASKARAN-THESIS-2016.pdf (1.638Mb)
      Date
      2016-12-19
      Author
      G Baskaran, Manoj Nair 1984-
      Type
      Thesis
      Degree Level
      Masters
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      Abstract
      Various airway inflammatory diseases progress as a result of chronic inflammation, specifically neurogenic inflammation. One factor known to be involved in this is RAGE. This work has established RAGE as a key mediator in LPS-induced sensitization of afferent thoracic dorsal root ganglia neurons of the upper airway in mice. This was demonstrated from electrophysiological data showing the failure of LPS to potentiate capsaicin-evoked TRPV1 currents and increase action potential generation in RAGE KO relative to wild-type thoracic neurons. Mass spectrometry analysis for protein identification represents a step forward in biochemistry, allowing to obtain a global picture of the proteins expressed in a cell, including isoforms, post-translational modifications and downstream signalling pathways.
      Degree
      Master of Science (M.Sc.)
      Department
      Physiology
      Program
      Physiology
      Supervisor
      Campanucci, Veronica; Ianowski, Juan
      Committee
      Mulligan, Sean; Chlan-Fourney, Jennifer; Cayabyab, Francisco
      Copyright Date
      December 2016
      URI
      http://hdl.handle.net/10388/7630
      Subject
      RAGE, RAGE isoforms, RAGE KO, thoracic dorsal root ganglia, LPS, airway inflammatory disease, neurogenic inflammation, sensory neuron sensitization
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