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dc.contributor.advisorSingh, Jaswant
dc.creatorCaunce, Serena L
dc.date.accessioned2017-08-03T17:11:18Z
dc.date.available2017-08-03T17:11:18Z
dc.date.created2017-10
dc.date.issued2017-08-03
dc.date.submittedOctober 2017
dc.identifier.urihttp://hdl.handle.net/10388/7992
dc.description.abstractThe main objective of this thesis was to develop a three-dimensional method to assess ovarian structures and relate their vascularity to their function (i.e. hormone production, oocyte quality, ovulatory capacity, etc.). This novel method uses video segments recorded after free-hand movement of the routine linear-array transducer, obtained vascularity index values are independent of speed of transducer movement, and the method does not require a-priori operator selection of images, i.e. is an objective method. In Study 1, colour Doppler ultrasonography was used to assess the ovaries of water buffalo (Bubalus bubalis) who were induced to ovulate using a gonadotropin releasing hormone analogue. Ultrasonographic examinations were performed daily to assess ovulation and subsequent corpus luteum (CL) development. Recorded cineloops were exported from the ultrasound machine and analyzed on a personal computer equipped with imaging software from two sources, Fiji (ImageJ) and Imaris. Fiji was used to perform conventional two-dimensional image analysis and Imaris was used to develop a new three-dimensional analysis method that does not require operator selection of images (i.e. objective method). Verification of the three-dimensional method was performed using two-dimensional data. Buffalo that did not ovulate within 24 hours of artificial insemination (40h from time of gonadotrophin releasing hormone analogue treatment) (n=3) tended to have lower change in vascular flow (P=0.06) to their preovulatory follicles when compared to those that ovulated (n=13) using the three-dimensional analysis method. In Study 2, power Doppler ultrasonography was used to assess the ovaries of 4-month-old prepubertal beef heifers (Bos taurus) who were superstimulated using two follicle stimulating hormone (FSH) protocols. Ultrasonographic scans were performed on the day of luteinizing hormone (LH) treatment, the day of oocyte collection, and the third and seventh day following follicular aspiration. The three-dimensional method developed in Study 1 was used to calculate vascularity indices for the four time points. Ovarian vascularity was then related to the expansion of the cumulus-oocyte-complexes that were retrieved, as well as the subsequent luteal tissue that developed following follicle aspiration. Luteal vascularity index measured at three days following follicular aspiration were related to the rise in plasma progesterone concentration over time (from 24 h following LH to day seven after aspiration) (r=0.65, P<0.01). Prepubertal calves demonstrated an increase in ovarian vascularity index under the influence of exogenous LH that was similar to adult animals. Additionally, an increased duration of superstimulation (seven days instead of four) was important for calves in the development of luteal tissue on day seven, luteal vascularity and progesterone production. In conclusion, three-dimensional image analysis of colour Doppler ultrasound images was superior to two-dimensional in detecting follicles that did not ovulate. This method using power Doppler ultrasound images was also applied to prepubertal calves undergoing superstimulation and found that luteal vascular flow was related to progesterone production following follicular aspiration.
dc.format.mimetypeapplication/pdf
dc.subjectcolour Doppler ultrasonography
dc.subjectthree-dimensional volumetric analysis
dc.subjectovary
dc.subjectbuffalo
dc.subjectreproduction
dc.subjectfollicle
dc.subjectblood-flow
dc.subjectvascularity
dc.subjectCL
dc.subjectprepubertal cattle
dc.subjectcalf reproduction
dc.subjectovarian vascularity
dc.subjectFSH stimulation
dc.subjectfollicle development
dc.subjectluteal development
dc.subjectcumulus-oocyte-complex
dc.subjectovum pick-up
dc.titleTHREE-DIMENSIONAL ANALYSIS OF COLOUR DOPPLER ULTRASOUND IMAGES: THE RELATIONSHIP OF VASCULAR FLOW TO OVARIAN STRUCTURES AND THEIR FUNCTION IN BUFFALO COWS AND BEEF CALVES
dc.typeThesis
dc.date.updated2017-08-03T17:11:18Z
thesis.degree.departmentVeterinary Biomedical Sciences
thesis.degree.disciplineVeterinary Biomedical Sciences
thesis.degree.grantorUniversity of Saskatchewan
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (M.Sc.)
dc.type.materialtext
dc.contributor.committeeMemberAdams, Gregg P
dc.contributor.committeeMemberCard, Claire
dc.contributor.committeeMemberHonaramooz, Ali


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