University of SaskatchewanHARVEST
  • Login
  • Submit Your Work
  • About
    • About HARVEST
    • Guidelines
    • Browse
      • All of HARVEST
      • Communities & Collections
      • By Issue Date
      • Authors
      • Titles
      • Subjects
      • This Collection
      • By Issue Date
      • Authors
      • Titles
      • Subjects
    • My Account
      • Login
      JavaScript is disabled for your browser. Some features of this site may not work without it.
      View Item 
      • HARVEST
      • Electronic Theses and Dissertations
      • Graduate Theses and Dissertations
      • View Item
      • HARVEST
      • Electronic Theses and Dissertations
      • Graduate Theses and Dissertations
      • View Item

      Effect of Bacteria on Airway Submucosal Glands Liquid Secretion in Swine

      Thumbnail
      View/Open
      LUAN-DISSERTATION-2018.pdf (4.696Mb)
      Date
      2018-01-15
      Author
      Luan, Xiao Jie 1987-
      Type
      Thesis
      Degree Level
      Doctoral
      Metadata
      Show full item record
      Abstract
      Cystic fibrosis (CF) is a genetic disorder caused by mutations in the gene encoding for the cystic fibrosis transmembrane conductance regulator (CFTR) anion channel. Currently, more than 4,100 Canadians have CF. The major cause of CF morbidity and mortality is airway disease, for which there is no cure. The events leading from CFTR gene mutation to CF airway disease are not fully understood, and there is controversy regarding the primary defect responsible for CF airway disease pathogenesis. Newborn CFTRΔF508/ΔF508 and CFTR-/- swine show no sign of infection and inflammation in the lung but suffer from defective bacteria eradication caused by abnormal innate immune system. The cornerstone of the airway’s innate immune defense is mucociliary clearance, which relies on the normal regulation of airway surface liquid (ASL), which covers the airway epithelium. It has been hypothesized that abnormal ASL is the primary defect that leads to the failure of the airway innate immune defense in CF. Evidence show that the airway submucosal gland functions abnormally in both CF patients and in animal models of CF. This is not surprising since airway submucosal glands normally express CFTR. However, the function of the gland in health and disease is not fully understood. The response of airway submucosal gland to inhaled bacteria has never been tested and its ion transport properties have not been fully described. Our objective is to investigate the effect of inhaled bacteria on airway submucosal gland secretion, and to study and compare the function of different segments of airway submucosal gland in wild-type and CF airway. Knowledge generated by this thesis would help better understand CF airway pathophysiology and may contribute to improving treatment methods.
      Degree
      Doctor of Philosophy (Ph.D.)
      Department
      Physiology
      Program
      Physiology
      Supervisor
      Ianowski, Juan P.; Chapman, Dean
      Committee
      Fisher, Thomas E.; Lee, Paul; Aulakh, Gurpreet K.
      Copyright Date
      January 2018
      URI
      http://hdl.handle.net/10388/8348
      Subject
      Cystic fibrosis
      Airway surface liquid
      Submucosal gland
      Pseudomonas aeruginosa
      Synchrotron imaging
      Collections
      • Graduate Theses and Dissertations
      University of Saskatchewan

      University Library

      © University of Saskatchewan
      Contact Us | Disclaimer | Privacy