Dark septate endophytic (DSE) fungi represent a diverse group of root-colonizing fungal species that are common in environments with strong abiotic stress, such as semiarid prairie regions where their abundance in roots can exceed mycorrhizal fungi. Some DSE fungal species have the ability to benefit host plant growth under water stress conditions. Here we tested the effects of 49 DSE species on chickpea biomass growing under water limiting condition. Three DSE fungal species including Hypocrea lixii, Geomyces vinaceus and Mortierella alpina significantly increased the biomass of chickpea. However the majority of the DSE species did not significantly affect plant biomass and some species decreased that.