Fungal surface measurements: water contact angles

Abstract

Fungal surface properties have been implicated as one of the main factors affecting fungal colonization and adhesion to plant surfaces. Characterization of fungal surfaces through hydrophobic measurements is important for understanding its function. Water contact angles are a direct and simple approach for characterization of fungal surface hydrophobicity. The objective of this study was to evaluate if utilization of undisturbed fungal cultures coupled with versatile image analysis allow for more accurate contact angle measurements. Fungal cultures were grown on agar slide media and contact angles were measured utilizing a modified microscope and digital camera setup, with Low Bond Axisymmetric Drop Shape Analysis Model (LB_ADSA) for contact angle determination. Fungal strains were categorized into hydrophobic, hydrophilic and a newly defined hydroamphiphilic class containing fungi taxa with changing hydrophobicity.