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      • HARVEST
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      EFFECT OF VARYING DIETARY VITAMIN A SUPPLEMENTATION LEVELS IN COMBINATION WITH ADH1C GENOTYPE ON INTRAMUSCULAR FAT DEPOSITION IN FINISHING BEEF STEERS

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      KRONE-THESIS.pdf (1.291Mb)
      Date
      2014-07-16
      Author
      Krone, Kristin
      Type
      Thesis
      Degree Level
      Masters
      Metadata
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      Abstract
      Previously, ADH1Cc.-64T>C was shown to have an association with intramuscular fat (IMF) in the longissimus thoracis (LT) muscle when vitamin A was limited in finishing rations of beef steers. The purpose of the current study was to determine the optimum vitamin A supplementation level, in combination with ADH1C genotype, to increase IMF of the LT muscle. Forty-five TT, 45 CT and 27 CC cross-bred steers, black in colour, were backgrounded on a commercial ration containing 3360 IU vitamin A/kg DM. During finishing the steers were randomly assigned to one of three vitamin A treatments at 25, 50 and 75% of the NRC recommendation of 2200 IU/kg DM. Treatments were administered via an oral bolus. Carcass quality was evaluated and a sample from the LT muscle was collected for analysis of IMF. A treatment x genotype interaction (P=0.04) was observed for IMF; TT steers on the 75% treatment had higher IMF relative to CT and CC steers on the same treatment. Intramuscular fat was also higher for TT steers on the 75% treatment in comparison to TT steers on the 25% treatment. Eighty-four percent of the steers graded Canada AAA. Western blot analysis showed that TT steers had higher (P=0.02) ADH1C levels in hepatic tissue. Previously, TT steers had increased IMF when fed limited vitamin A. In the current study the lack of variation between treatments and genotypes at the lower vitamin A treatment levels was likely due to the majority of the steers grading Canada AAA (USDA Choice). However, the western blot data supports that TT steers are expected to have higher IMF deposition, due to an increase production of ADH1C.
      Degree
      Master of Science (M.Sc.)
      Department
      Animal and Poultry Science
      Program
      Animal Science
      Supervisor
      Buchanan, Fiona C.; McKinnon, John J.
      Committee
      Hendrick, Steve; Hogan, Natacha
      Copyright Date
      June 2014
      URI
      http://hdl.handle.net/10388/ETD-2014-06-1580
      Subject
      Vitamin A
      beef cattle
      nutrigenetics
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      • Graduate Theses and Dissertations
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