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dc.contributor.advisorKaminskyj, Susanen_US
dc.creatorHe, Xiaoxiaoen_US
dc.date.accessioned2014-09-27T12:00:23Z
dc.date.available2014-09-27T12:00:23Z
dc.date.created2014-09en_US
dc.date.issued2014-09-26en_US
dc.date.submittedSeptember 2014en_US
dc.identifier.urihttp://hdl.handle.net/10388/ETD-2014-09-1735en_US
dc.description.abstractSystemic fungal infection is a life-threatening problem. Anti-fungal drugs are the most effective clinical strategy to cure such infections. However, most current anti-fungal drugs either have high toxicity or have a narrow spectrum of effect. Meanwhile, anti-fungal drugs are losing their clinical efficacy due to emerging drug resistance. To protect us from these deadly pathogenic fungi, scientists need to study new drug targets and to solve problems related to drug resistance. The cell wall is essential for fungal cell survival and is absent from animal cells, so it is a promising reservoir for screening safe and effective drug targets. Alpha-1,3-glucan is one of the major cell wall carbohydrates and is important for the virulence of several pathogenic fungi. In this thesis, molecular biology and microscopy techniques were used to investigate the function and the synthesis process of α-1,3-glucan in the model fungus A. nidulans. My results showed that α-1,3-glucan comprises about 15% of A. nidulans cell wall dry weight, but also that α-1,3-glucan does not have an important role in cell wall formation and cell morphology. Deletion of α-1,3-glucan only affects conidial adhesion and cell sensitivity to calcofluor white. In contast, elevated α-1,3-glucan content can cause severe phenotypic defects. To study the α-1,3-glucan synthesis process, I systematically characterized four proteins, including two α-1,3-glucan synthases (AgsA and AgsB) and two amylase-like proteins (AmyD and AmyG). Results showed AgsA and AgsB are both functional synthases. AgsB is the major synthase due to its constant expression. AgsA mainly functions in conidiation stages. AmyG is a cytoplasmic protein that is critical for α-1,3-glucan synthesis, likely being required for an earlier step in the synthesis process. In contrast to the other three proteins, AmyD has a repressive effect on α-1,3-glucan accumulation. These results shed light on therapeutic strategies that might be developed against α-1,3-glucan. I also developed a strategy to investigate drug resistance mutations. The tractability of A. nidulans and the power of next generation sequencing enabled an easy approach to isolate single mutation strains and to identify the causal mutations from a genome scale efficiently. I suggest this strategy has applications to study the drug resistance mechanisms of current anti-fungal drugs and even possibly future ones.en_US
dc.language.isoengen_US
dc.subjectAspergillus nidulansen_US
dc.subjectalpha-1,3-glucanen_US
dc.subjectcell wallen_US
dc.subjectdrug resistanceen_US
dc.subjectalpha-1,3-glucan synthaseen_US
dc.subjectanti-fungal drugen_US
dc.titleUsing Aspergillus nidulans to study alpha-1,3-glucan synthesis and the resistance mechanism against cell wall targeting drugsen_US
thesis.degree.departmentBiologyen_US
thesis.degree.disciplineBiologyen_US
thesis.degree.grantorUniversity of Saskatchewanen_US
thesis.degree.levelDoctoralen_US
thesis.degree.nameDoctor of Philosophy (Ph.D.)en_US
dc.type.materialtexten_US
dc.type.genreThesisen_US
dc.contributor.committeeMemberAndres, Joseen_US
dc.contributor.committeeMemberSanders, Daviden_US
dc.contributor.committeeMemberCavarlho, Carlosen_US
dc.contributor.committeeMemberWei, Yangdouen_US


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