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dc.contributor.advisorFowler, Brianen_US
dc.contributor.advisorChibbar, Ravindra N.en_US
dc.creatorDenesik, Tyrel Jonathanen_US
dc.date.accessioned2007-04-01T20:56:44Zen_US
dc.date.accessioned2013-01-04T04:27:58Z
dc.date.available2007-04-02T08:00:00Zen_US
dc.date.available2013-01-04T04:27:58Z
dc.date.created2007-04en_US
dc.date.issued2007-04-02en_US
dc.date.submittedApril 2007en_US
dc.identifier.urihttp://hdl.handle.net/10388/etd-04012007-205644en_US
dc.description.abstractWinter wheat (Triticum aestivum L.), seeded in the fall, cold acclimates when exposed to low fall temperatures. Growth resumes in spring, culminating in early summer harvest. Winter wheat yield is generally 20-25% higher than spring wheat. However, winter damage/kill can reduce its yield. A better understanding of the cold acclimation/tolerance process could help in the development of improved breeding strategies for winter wheat hardiness. Transcriptional activators and specific cold regulated (COR) genes are induced as a result of exposure to low temperatures. Thus, the objective of this study was to determine the quantitative expression of three COR genes (Wcs120, Wcor410 and Wcor14b) and one transcriptional activator (WCBF1) in field-grown wheat using real-time PCR and to establish any association with LT50 (temperature at which 50% of plants are killed). Winter Norstar (vrn-A1/vrn-A1), spring Manitou (Vrn-A1/Vrn-A1) and two near-isogenic lines (Spring Norstar (Vrn-A1/vrn-A1) and Winter Manitou (vrn-A1/vrn-A1), respectively) were used in these studies. Plants were sampled on three dates (Sept. 29, Oct. 12 and Oct. 26) in the fall of 2004. Accumulation of WCBF1 transcripts was highest in Norstar, but in all four genotypes there was an increase in transcripts by the second sampling date, followed by a decline on the third sampling date. Wcs120 transcripts increased from the first to the third sampling date in Norstar, Spring Norstar and Winter Manitou, but increased to the second sampling date and decreased by the third in Manitou. For Wcor14b, generally there was an increase to the second sampling date, followed by a decrease or steady levels on the third. Wcor410 showed a similar pattern, except for Spring Norstar wherein transcript levels increased by the third sampling date. With the exception of Wcor410 in Manitou, the Vrn-A1 locus affected gene expression in all genotypes. However, only Wcs120 expression followed the low-temperature tolerance pattern in these genotypes.en_US
dc.language.isoen_USen_US
dc.subjectWcor410en_US
dc.subjectWCBF1en_US
dc.subjectWcor14ben_US
dc.subjectreal-time PCRen_US
dc.subjectwheaten_US
dc.subjectcold toleranceen_US
dc.subjectWcs120en_US
dc.subjectgene expression analysisen_US
dc.titleQuantitative expression analysis of four low-temperature-tolerance-associated genes during cold acclimation in wheat (Triticum aestivum L.)en_US
thesis.degree.departmentPlant Sciencesen_US
thesis.degree.disciplinePlant Sciencesen_US
thesis.degree.grantorUniversity of Saskatchewanen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Science (M.Sc.)en_US
dc.type.materialtexten_US
dc.type.genreThesisen_US
dc.contributor.committeeMemberVan Kessel, Andrew G.en_US
dc.contributor.committeeMemberPozniak, Curtis J.en_US
dc.contributor.committeeMemberGray, Gordon R.en_US
dc.contributor.committeeMemberCoulman, Bruce E.en_US


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