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dc.contributor.advisorSanders, David A. R.en_US
dc.contributor.advisorPalmer, Daviden_US
dc.creatorToogood, Ronald Danielen_US
dc.date.accessioned2009-04-09T16:16:57Zen_US
dc.date.accessioned2013-01-04T04:28:28Z
dc.date.available2010-04-15T08:00:00Zen_US
dc.date.available2013-01-04T04:28:28Z
dc.date.created2009en_US
dc.date.issued2009en_US
dc.date.submitted2009en_US
dc.identifier.urihttp://hdl.handle.net/10388/etd-04092009-161657en_US
dc.description.abstractThe thesis presented here describes the steps that were taken in an attempt to solve the protein structure of MenD via molecular replacement and multiple wavelength anomalous dispersion. The introduction provides background on menaquinone biosynthesis and the role of MenD in this metabolic pathway. Also, a detailed discussion of the DC Family of enzymes, a subgroup of ThDP dependent enzymes, which MenD is a part of, is included. Utilizing various software packages a 1.9 Å data set was processed and analyzed in an attempt to provide a molecular replacement result. When molecular replacement was deemed incapable of solving the phase problem of the data set, the production of SeMet protein was attempted to allow for MAD phasing. A homology model of MenD was produced using the program Modeller with benzaldehyde lyase as a template. A structure based sequence alignment was done with all DC Family enzymes with structures published. Then a second structure based sequence alignment was done to compare the same set to the Modeller model. This was done to gain a deeper understanding of MenD and how it interacts with its cofactors ThDP and Mg2+. Furthermore, these results were used to implicate potential active site residues.en_US
dc.language.isoen_USen_US
dc.subjectenzymeen_US
dc.subjectThDPen_US
dc.subjectSEPHCHCen_US
dc.subjectMenDen_US
dc.subjectX-ray crystallographyen_US
dc.titleStructural studies of MenD : a crystallographic endeavoren_US
thesis.degree.departmentChemistryen_US
thesis.degree.disciplineChemistryen_US
thesis.degree.grantorUniversity of Saskatchewanen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Science (M.Sc.)en_US
dc.type.materialtexten_US
dc.type.genreThesisen_US


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