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dc.contributor.advisorTanaka, Takujien_US
dc.creatorYang, Soo Inen_US
dc.date.accessioned2007-04-14T12:38:03Zen_US
dc.date.accessioned2013-01-04T04:29:07Z
dc.date.available2008-04-23T08:00:00Zen_US
dc.date.available2013-01-04T04:29:07Z
dc.date.created2007en_US
dc.date.issued2007en_US
dc.date.submitted2007en_US
dc.identifier.urihttp://hdl.handle.net/10388/etd-04142007-123803en_US
dc.description.abstractLactobacillus plantarum (Lb. plantarum) NRRL B4496 and Lactococcus lactis (Lc. lactis) NRRL B1821 prolidase genes were isolated, cloned, and sequenced. The sequence-confirmed genes were subcloned into the expression systems. The recombinant prolidases from the pKK223-3 systems were purified through ammonium sulphate precipitation and anion-exchange column chromatography. Recombinant Lb. plantarum prolidase, however, demonstrated a loss of activity during the purification. The following characterization work was performed on purified recombinant Lc. lactis prolidase. The mass spectroscopic result and the molecular modelling suggested a 80 kDa homodimer with two metal cations at the catalytic centre of the prolidase. The optimum temperature was 50 ºC and showed more than 50% activities between 40 and 55 ºC. The enzyme was most stable at 30 ºC and withstood 20 min of heat-treatment up to 60 ºC, however, lost activity over 70 ºC. Circular dichroism indicated a denaturation temperature of 67 ºC. The optimum pH was 6.5 for hydrolyzing Leu-Pro and the enzyme did not display any activity below pH 5.5 nor above pH 7 with this peptide. However, Phe-Pro was hydrolyzed the fastest at pH 7 and Arg-Pro had a maximum rate at pH 9. This metallopeptidase exhibited a broad range of metal cation preference, hydrolyzing Leu-Pro with Mn++, Co++, Zn++, Ca++, and Mg++. Further kinetic analysis showed unusual allostery of the enzyme (Hill coefficient: 1.3). The unique substrate intakes onGlu-Pro and tripeptides were observed while Val-Pro was not hydrolyzed. The molecular modelling of this prolidase suggested a difference in the substrate specificity resulting from a loop structure, L33 to R40, near the substrate binding site.en_US
dc.language.isoen_USen_US
dc.subjectLactic acid bacteriaen_US
dc.subjectPepQen_US
dc.subjectprolidaseen_US
dc.titleCloning, expression, and characterization of lactic acid bacteria recombinant prolidasesen_US
thesis.degree.departmentApplied Microbiology and Food Scienceen_US
thesis.degree.disciplineApplied Microbiology and Food Scienceen_US
thesis.degree.grantorUniversity of Saskatchewanen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Science (M.Sc.)en_US
dc.type.materialtexten_US
dc.type.genreThesisen_US
dc.contributor.committeeMemberShand, Phyllis J.en_US
dc.contributor.committeeMemberKorber, Darren R.en_US


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