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dc.contributor.advisorGordon, Johnen_US
dc.creatorXie, Siyuanen_US
dc.date.accessioned2010-05-19T17:20:19Zen_US
dc.date.accessioned2013-01-04T04:31:13Z
dc.date.available2011-05-26T08:00:00Zen_US
dc.date.available2013-01-04T04:31:13Z
dc.date.created2010-05en_US
dc.date.issued2010-05en_US
dc.date.submittedMay 2010en_US
dc.identifier.urihttp://hdl.handle.net/10388/etd-05192010-172019en_US
dc.description.abstractMultiple sclerosis is a chronic autoimmune neurological disease characterized by inflammatory cell infiltration and demyelination in the central nervous system (CNS). It is considered to be mediated by Th1 and Th17 immune responses. Experimental autoimmune encephalomyelitis (EAE) is widely used as a mouse model to study MS as it has features and histopathology similar to that of MS. Tolerogenic dendritic cells (DC) are reported to efficiently prevent sensitization for EAE. In this research, we induced tolerogenic DC (DC10) by differentiating them with IL-10. Compared to immature DC, DC10 did not show increased expression of MHC II or the co-stimulatory molecules CD40, CD80 and CD86, and produced low levels of pro-inflammatory cytokines IL-1â, IL-6, and IL-12 but higher levels of IL-10. This is consistent with their possessing a tolerogenic phenotype. We found that three intraperitoneal (i.p.) injections of DC10 successfully inhibited the signs of established, ongoing EAE: DC10 significantly reduced the clinical scores, demyelination and cell infiltration in the spinal cord, as well as the production of IL-4, IL-6, IL-10, IL-17 and IFN-ã by spleen and lymph node (LN) lymphocytes. DC10 treatments did not significantly affect inflammatory cytokine mRNA levels in the CNS. We found that there was higher FoxP3 expression in the CNS in response to DC10 treatments relative to PBS-treated animals. We also found that DC10 treatments significantly enhanced IgG1, IgG2a and IgG2b production and total spleen and LN lymphocyte proliferation following challenge with myelin oligodendrocyte glycoprotein (MOG) antigen. As far as we know, this is the first report showing the successful therapeutic treatment with tolerogenic DC10 of established EAE in mice.en_US
dc.language.isoen_USen_US
dc.subjectmultiple sclerosisen_US
dc.subjectEAEen_US
dc.subjecttoleranceen_US
dc.subjectdendritic cellen_US
dc.subjectCNSen_US
dc.subjectinflammationen_US
dc.titleIL-10-differentiated dendritic cells treatment for Experimental Autoimmune Encephalomyelitis (EAE), a model of human Multiple Sclerosisen_US
thesis.degree.departmentVeterinary Microbiologyen_US
thesis.degree.disciplineVeterinary Microbiologyen_US
thesis.degree.grantorUniversity of Saskatchewanen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Science (M.Sc.)en_US
dc.type.materialtexten_US
dc.type.genreThesisen_US
dc.contributor.committeeMemberGerdts, Volkeren_US
dc.contributor.committeeMemberTabel, Henryen_US
dc.contributor.committeeMemberMisra, Vikramen_US
dc.contributor.committeeMemberSingh, Baljiten_US


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