University of SaskatchewanHARVEST
  • Login
  • Submit Your Work
  • About
    • About HARVEST
    • Guidelines
    • Browse
      • All of HARVEST
      • Communities & Collections
      • By Issue Date
      • Authors
      • Titles
      • Subjects
      • This Collection
      • By Issue Date
      • Authors
      • Titles
      • Subjects
    • My Account
      • Login
      JavaScript is disabled for your browser. Some features of this site may not work without it.
      View Item 
      • HARVEST
      • Electronic Theses and Dissertations
      • Graduate Theses and Dissertations
      • View Item
      • HARVEST
      • Electronic Theses and Dissertations
      • Graduate Theses and Dissertations
      • View Item

      Plasma membrane proteins from herbicide resistant and susceptible wild oat biotypes

      Thumbnail
      View/Open
      Phelps_Sherrilyn_Marie_1999_sec.pdf (126.3Mb)
      Date
      1998
      Author
      Phelps, Sherrilyn Marie
      Type
      Thesis
      Degree Level
      Masters
      Metadata
      Show full item record
      Abstract
      The wild oat biotype UM1 is resistant to the aryloxyphenoxypropanoate (AOPP) and cyclohexanedione (CHD) classes of herbicides. When this project was initiated, the mechanism of herbicide resistance was unknown, but evidence suggested the possible involvement of plasma membrane proteins in the resistance mechanism. Comparison of membrane protein profiles of untreated resistant (UM1) and susceptible (UM5) plants showed no differences between the two biotypes. However, upon herbicide treatment a membraneassociated protein of 47 kDa was greatly reduced in amounts in the susceptible, but not resistant, biotype. This response was thought to be linked to the resistance mechanism. Further studies showed the resistance mechanism to involve an insensitive form of the target enzyme (acetyl CoA carboxylase), thereby negating the involvement of the plasma membrane protein effects in the resistance mechanism. Further characterization of the 47 kDa protein indicated that is was the large subunit of RUBISCO. The effect of AOPP and CHD herbicides on RUBISCO is not believed to be a secondary mode of action of these herbicides, but is related to the general onset of senescence induced by herbicide treatment in susceptible biotypes. The protein response was also inducible in the resistant biotype by treating with other herbicides to which this biotype was sensitive. Although the differential membrane protein response was not involved as a resistance mechanism, its depletion may be useful for detection of herbicide resistance in weed species.
      Degree
      Master of Science (M.Sc.)
      Department
      Plant Sciences
      Program
      Plant Sciences
      Supervisor
      Devine, Malcolm
      Committee
      Gusta, L.; Scoles, G.
      Copyright Date
      1998
      URI
      http://hdl.handle.net/10388/etd-05242012-083401
      Collections
      • Graduate Theses and Dissertations
      University of Saskatchewan

      University Library

      The University of Saskatchewan's main campus is situated on Treaty 6 Territory and the Homeland of the Métis.

      © University of Saskatchewan
      Contact Us | Disclaimer | Privacy