Cyclolinopeptides in flaxseed and flaxseed products
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Cyclolinopeptides (CLPs), a group of naturally occurring, hydrophobic, cyclic peptides in flax, have attracted a great deal of attention due to their immunosuppressive activity. The purpose of this project was to increase our understanding of the occurrence of CLPs in flaxseed, flaxseed tissues and flaxseed products. In the first study, systematic methods for CLP extraction, isolation, detection and quantification were developed. The solubility of CLPs in acetone led to its use as a preferred solvent for extraction of CLPs and other hydrophobic compounds from whole flaxseed. Solid phase extraction with a silica gel column followed by selective elution with organic solvents of increasing polarity enabled the isolation of a crude peptide-rich fraction. Reverse phase HPLC chromatography of peptide-rich fractions provided a method for separation and quantification of CLPs. In the second study, the levels of CLPs in cultivars of flaxseed were studied to determine if there was any impact of flax genotype or environment on peptide levels. The concentration of total CLPs varied from 189 Âµg/g (Flanders) to 303 Âµg/g (Somme) in the cultivars tested. Environment, cultivar and their interaction affected the observed concentration of CLPs. In the third study, the concentrations of CLPs in fractions produced from flaxseed were measured by HPLC in seed coat, cotyledon and oil bodies. The concentration of CLPs was higher in the cotyledon than in the seed coat. The highest CLP concentrations were found in the oil bodies. In the fourth study, CLP levels in flaxseed oil were measured during and after oil extraction and refining. The concentration of CLPs was higher in expeller-extracted crude oil and solid foots and lower in flaxseed meal. A comparison of CLP levels in flaxseed oil extracted with a small expeller and in commercially-produced flaxseed oil was performed. Crude flaxseed oil produced with a small expeller had higher levels of peptides than were observed in commercial flaxseed oil available at a local retail health food store. The effect of oil refining processes, including acid degumming and alkali refining on CLP stability, was studied. Acid degumming using 1% H3PO4 effectively removed all CLPs. Alkali refining was also demonstrated as being effective at decreasing levels of CLPs, although it failed to remove all peptides.
DegreeMaster of Science (M.Sc.)
DepartmentApplied Microbiology and Food Science
ProgramApplied Microbiology and Food Science
Copyright DateMay 2011