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      • HARVEST
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      Amelioration of experimental allergic encephalomyelitis (eae) by phase 2 enzyme inducer

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      AmeliorationOfEAEbyPhase2EnzymeInducer.pdf (3.861Mb)
      Date
      2010-06
      Author
      Yunus, Mohammed
      Type
      Thesis
      Degree Level
      Masters
      Metadata
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      Abstract
      The pathology of multiple sclerosis (MS) is characterized by an inflammatory mononuclear infiltration in the white matter. There has been converging evidence of the oxidative stress playing a role in the onset and progression of MS. We postulated that the decreasing oxidative stress might help in the management of MS. We know that the induction of phase 2 enzymes decreases the oxidative stress. The experimental allergic encephalomyelitis (EAE) induced in the Lewis rats were used to test this hypothesis. The 24 animals were placed into two groups: 1) those on normal rat chow, 2) those on rat chow containing 7.5 g/kg of tetra-butylhydroxyanisole (BHA), a food preservative. All the animals were administered 100 µg of guinea pig myelin basic protein in their tails to induce EAE and examined daily in a double blinded fashion. On 29th day of the induction, the animals were sacrificed, blood collected for glutathione (GSH) measurements and tissues collected for histology. All the animals, regardless of their diet status, developed symptoms of EAE on different days ranging from tail weakness to hind limb paralysis and all of them reached remission of acute EAE before the 28th day of induction. The non-BHA fed animals developed hind limb weakness in 8 animals and hind limb paralysis in 4 cases, while that of BHA fed group developed tail paralysis in 2, hind limb weakness in 2 and hind limb paralysis in 8 cases. The histology of the non-BHA group correlated well with the clinical symptoms of perivascular mononuclear infiltration. However, the BHA group revealed complete pathological recovery. Animals with BHA in the diet had significantly raised GSH, indicating the induction of phase 2 enzymes. We conclude that dietary phase 2 enzyme inducers show potential therapeutic benefits in EAE and should be examined for this role in MS.
      Degree
      Master of Science (M.Sc.)
      Department
      Pathology
      Program
      Pathology
      Supervisor
      Mohamed, Adel
      Committee
      Muir, Gillian; Krahn, John; Qureshi, Mabood; Kalra, Jawahar (Jay)
      Copyright Date
      June 2010
      URI
      http://hdl.handle.net/10388/etd-06232010-172753
      Subject
      nfkb
      ros
      cytokine
      T helper lymphocyte
      autoimmune
      oxidative stress
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