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dc.contributor.advisorTaylor, Janeten_US
dc.creatorGibson, Shawnen_US
dc.date.accessioned2005-08-22T11:50:50Zen_US
dc.date.accessioned2013-01-04T04:53:48Z
dc.date.available2005-08-22T08:00:00Zen_US
dc.date.available2013-01-04T04:53:48Z
dc.date.created2005-07en_US
dc.date.issued2005-07-06en_US
dc.date.submittedJuly 2005en_US
dc.identifier.urihttp://hdl.handle.net/10388/etd-08222005-115050en_US
dc.description.abstractA cDNA sequence representing a Brassica carinata gene the expression of which is induced by copper chloride treatment, was isolated from a library constructed with mRNA from treated leaves, and designated CIL1 (COPPER CHLORIDE INDUCED in LEAVES). A Basic Local Alignment Search Tool search revealed that CIL1 has similarities to an auxin-induced gene, AIR12 from Arabidopsis thaliana. Southern blot analysis of CIL1 in B. carinata, B. nigra and B. oleracea indicated that it is a member of a small multigene family. Antisense CIL1 transgenic plants were generated to investigate the function of CIL1, and the resulting transformants displayed increased secondary branching suggesting that CIL1 has a role in regulating hormone content or plant architecture. Results of induction studies indicate that the auxin analog a-naphthalene acetic acid, the cytokinin 6-benzylaminopurine, and +/- abscisic acid increase expression of CIL1. Seven CIL1 antisense lines were grown to the T4 generation and were confirmed homozygous. Analysis of CIL1 expression using real-time quantitative RT-PCR showed reduced expression in every examined line. Transgenic plants produced many leaves at the lateral meristems indicating a release of apical dominance. Additionally, the concentrations of auxins, cytokinins, and abscisic acid were altered in the roots and stems of transgenic plants compared to non-transformed plants. Therefore, CIL1 has a role in regulating hormone content that affects lateral meristem activity, apical dominance, and leaf production.en_US
dc.language.isoen_USen_US
dc.subjectcytokininen_US
dc.subjectcatecholamineen_US
dc.subjectphytohormoneen_US
dc.subjectauxinen_US
dc.titleThe role of CIL1 in brassica carinata lateral meristem developmenten_US
thesis.degree.departmentBiologyen_US
thesis.degree.disciplineBiologyen_US
thesis.degree.grantorUniversity of Saskatchewanen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Science (M.Sc.)en_US
dc.type.materialtexten_US
dc.type.genreThesisen_US
dc.contributor.committeeMemberWilson, Kenneth E.en_US
dc.contributor.committeeMemberSawhney, Vipen K.en_US
dc.contributor.committeeMemberBonham-Smith, Peta C.en_US


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