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dc.contributor.advisorPalmer, David R. J.en_US
dc.creatorLangill, David Mitchellen_US
dc.date.accessioned2010-09-21T20:21:12Zen_US
dc.date.accessioned2013-01-04T04:59:40Z
dc.date.available2011-09-27T08:00:00Zen_US
dc.date.available2013-01-04T04:59:40Z
dc.date.created2010en_US
dc.date.issued2010en_US
dc.date.submitted2010en_US
dc.identifier.urihttp://hdl.handle.net/10388/etd-09212010-202112en_US
dc.description.abstract3,3'-Neotrehalosadiamine (NTD) is a diaminosugar that possesses a rare alpha,beta-1,1'-linked glycosidic bond and has been reported to possess antimicrobial activity against Staphylococcus aureus. The ntdABC operon contains three structural genes that are necessary for the production of NTD in certain mutants of Bacillus subtilis. The gene predicted to be the first in the NTD biosynthetic pathway, ntdC, was subcloned into pET-28b as the hexa-histidine tagged fusion. The gene product was expressed, purified to homogeneity, and found to be an NAD+-dependent glucose 6-phosphate 3-dehydrogenase, likely operating according to a ternary complex mechanism and possessing a catalytic dyad composed by D176 and H180. The advent of this knowledge suggests that additional genes are required for the biosynthesis of NTD aside from the three encoded by the ntdABC operon.en_US
dc.language.isoen_USen_US
dc.subjectDehydrogenaseen_US
dc.subjectAntibioticen_US
dc.subjectNeotrehalosadiamineen_US
dc.subjectNtdCen_US
dc.subjectBiochemistryen_US
dc.subjectOrganic synthesis; Glycosylations; Aminosugaren_US
dc.subjectEnzymologyen_US
dc.titleSynthesis of neotrehalose; kinetics and mutagenesis of NtdCen_US
thesis.degree.departmentChemistryen_US
thesis.degree.disciplineChemistryen_US
thesis.degree.grantorUniversity of Saskatchewanen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Science (M.Sc.)en_US
dc.type.materialtexten_US
dc.type.genreThesisen_US
dc.contributor.committeeMemberForsyth, Georgeen_US
dc.contributor.committeeMemberSteer, Ronald P.en_US
dc.contributor.committeeMemberSanders, David A. R.en_US
dc.contributor.committeeMemberWard, Dale E.en_US


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