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      Inheritance and linkage of morphological, isozyme and RAPD markers in grasspea

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      Date
      1997-04-01
      Author
      Chowdhury, Mahboob Alam
      Type
      Thesis
      Degree Level
      Doctoral
      Metadata
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      Abstract
      Experiments were conducted to determine the outcrossing rate, the inheritance of markers and establish a basic linkage map in grasspea, Lathyrus sativus L. The outcrossing rate in a white-flowered line of grasspea ranged from 1.7 to 2.7% among eight combinations of gene frequency and location. The outcrossing rate in this study (2.2 ± 0.7%) suggests that individual lines of grasspea should be maintained in isolation to maintain their genetic integrity. Inheritance and linkage were determined for one morphological, 11 isozyme and 72 RAPD markers in five F2 populations (all RAPD markers were in one F2 population). The inheritance of flower colour was monogenic with colour dominant over white. The isozymes, ACO-1, ACO-2, AAT-1, AAT-2, EST-6, FDH, LAP-1, PGD-2, SKDH and TPI-1, were codominantly expressed with monogenic inheritance. The isozymes LAP-1 and PGD-2 segregated in a non-Mendelian ratios in the crosses PI 426891.1.3 x PI 283564c.3.2 and PI 426891.1 x PI 172930.4, respectively. The isozymeEST-3 was monogenically inherited and dominantly expressed. Most RAPD markers segregated in a 3:1 ratio. Marker UBC368425/655 segregated in a co-dominant fashion. The RAPD markers UBC304831, UBC304964, UBC308990, UBC3221432, UBC328831, UBC3321118, UBC33211581, UBC333617, UBC349752
      Degree
      Doctor of Philosophy (Ph.D.)
      Department
      Plant Sciences
      Program
      Plant Sciences
      Committee
      Slinkard, Alfred
      Copyright Date
      April 1997
      URI
      http://hdl.handle.net/10388/etd-10212004-000651
      Subject
      RAPD markers
      outcrossing rate
      Lathyrus sativus L.
      grasspea
      legumes
      plant genetics
      genetic linkage map
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      • Graduate Theses and Dissertations
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