University of SaskatchewanHARVEST
  • Login
  • Submit Your Work
  • About
    • About HARVEST
    • Guidelines
    • Browse
      • All of HARVEST
      • Communities & Collections
      • By Issue Date
      • Authors
      • Titles
      • Subjects
      • This Collection
      • By Issue Date
      • Authors
      • Titles
      • Subjects
    • My Account
      • Login
      JavaScript is disabled for your browser. Some features of this site may not work without it.
      View Item 
      • HARVEST
      • Electronic Theses and Dissertations
      • Graduate Theses and Dissertations
      • View Item
      • HARVEST
      • Electronic Theses and Dissertations
      • Graduate Theses and Dissertations
      • View Item

      A sphenoidal mechanism of midfacial retrognathia in the brachyrrhine mouse

      Thumbnail
      View/Open
      nq24031.pdf (6.054Mb)
      Date
      1996-07-01
      Author
      Ma, Wenbin
      Type
      Thesis
      Degree Level
      Doctoral
      Metadata
      Show full item record
      Abstract
      Class III malocclusion in orthodontic patients typically results from midfacial retrognathia. However, the etiology of the midfacial retrognathia remains unclear. The cranial base is considered to play an important role in the emergence of midfacial morphology due to its location within the craniofacial region as well as its dramatic growth activity during the later prenatal and early postnatal periods. Previously, the nasal septum was considered a key cranial base component which functioned to pull the maxilla anteriorly during growth. Although this nasal septal theory of midfacial advancement is generally accepted, certain midfacial abnormalities occur in the presence of normal nasal septal morphology and growth indicating that additional craniofacial regions must contribute to the control of midfacial prognathism. The purpose of this study was to describe a mouse mutant which displays midfacial retrognathia and to delineate regions of cranial base malgrowth. Further, cellular growth mechanisms responsible for causing the abnormal cranial base growth trajectories were identified. Adult 3H1 Brachyrrhine (Br) male mice, displaying midfacial retrognathia with a characteristic Class III malocclusion, were bred to normal C3H females. Litters were examined to determine whether Br offspring could be distinguished from one another between Theiler stages 23 (E15) and 27 (E19) using qualitative and quantitative methods. Results showed that two distinct groups of offspring were derived: one with midfacial retrognathia and the other without. The cranial base of Br mutants displayed a malformed sphenoidal region while the nasal septum appeared much less affected as revealed by finite element morphometric analysis. In vivo autoradiographic analysis demonstrated the existence of temporal growth sites (TGS). TGS in the sphenoidal regions were deficient in the prenatal Br mouse. Using immunohistochemistry, insulin growth factor (IGF-I) and epidermal growth factor (EGF) as well as their receptors (IGF-IR and EGF-R) were found to be expressed in cranial base chondrocytes. In order to determine whether chondrocytic responses to these growth factors were deficient in the mutant, cell cultures were established, treated with IGF-I or EGF, and cellular proliferation and differentiation were measured using (3H) -thymidine and (35S) -sulfate incorporation. Results from this analysis showed that chondrocytes from the Br posterior cranial base were less responsive to EGF compared to cells from normal posterior cranial bases. Data from this study suggest that the Br mouse displays midfacial retrognathia in a heritable fashion. Deficient growth of the Br sphenoid, particularly in the presphenoidal and sphenoethmoid region, is crucial for the establishment of midfacial retrognathia. In the Br mouse, the sphenoidal deficiency is associated with depressed proliferation in TGS. IGF-I and EGF, as well as their receptors, are expressed within the murine cranial base and the depressed level of cellular proliferation in the Br sphenoidal region results, at least in part, from a diminished response to EGF. Based on this study, I propose a "sphenoidal mechanism of midfacial advancement" whereby the sphenoid actively propels the midface forward in order to achieve proper maxillary prognathism during later prenatal and early postnatal period.
      Degree
      Doctor of Philosophy (Ph.D.)
      Department
      Anatomy and Cell Biology
      Program
      Anatomy and Cell Biology
      Committee
      Lozanoff, Scott
      Copyright Date
      July 1996
      URI
      http://hdl.handle.net/10388/etd-10212004-000813
      Subject
      medicine
      cell biology
      anatomy
      face
      abnormalities
      genetics
      skull
      craniofacial
      Collections
      • Graduate Theses and Dissertations
      University of Saskatchewan

      University Library

      © University of Saskatchewan
      Contact Us | Disclaimer | Privacy