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dc.creatorRajapaksha, R. M. Chandani P.en_US
dc.date.accessioned2004-10-21T00:14:17Zen_US
dc.date.accessioned2013-01-04T05:04:20Z
dc.date.available1997-01-01T08:00:00Zen_US
dc.date.available2013-01-04T05:04:20Z
dc.date.created1997-01en_US
dc.date.issued1997-01-01en_US
dc.date.submittedJanuary 1997en_US
dc.identifier.urihttp://hdl.handle.net/10388/etd-10212004-001417en_US
dc.description.abstractNitrification is a key N transformation process which provides one of the major mineral N sources for biota in terrestrial ecosystems and influences atmospheric and hydrospheric chemistry via secondary reactions of its products. This study was conducted to assess the relative contribution of autotrophs and heterotrophs to nitrification activity across a cultivated and a pasture landscape. Shoulder and footslope complexes were chosen as experimental units and assays were carried out in soil obtained from randomly selected grid points (10) at each landscape position. Gross autotrophic and heterotrophic nitrification rates were determined in situ and in the laboratory using the 15N isotope pool dilution technique in conjunction with the biochemical block C2H2. Michaelis-Menten kinetics for NH4+ oxidation were quantified in soil slurries. Population densities of autotrophic nitrifiers were obtained by the MPN technique. Heterotrophic nitrifiers were isolated using two selective media and identified based on fatty acid profiles and taxonomic morphology. Total nitrification ranged from 3.8 to 4.9 ìg N g-1 soil d-1 at both sites but at low moisture conditions declined to 2.9 and 2.2 ìg N g-1 soil d-1 at the cultivated and the pasture landscape, respectively. Heterotrophic nitrification contributed 17-22% and 14-31% to the total nitrification at the cultivated and the pasture landscape, respectively. Athrobacter spp. were the predominant heterotrophic nitrifier among the soil isolates. The average Vmax of NH4+ oxidation approached a maximum of 35 ìg N g-1 soil d-1 at the cultivated landscape, whereas at the pasture landscape it remained below 20 ìg N g-1 soil d-1. Conversely, apparent Km values at the pasture landscape were approximately 2 to 20-fold higher than those of the cultivated landscape and varied from 16 to 165 ì M. Ammonium-oxidizers at both landscapes operate adaptive mechanisms toward the substrate fluxes and survive under oligotrophic conditions. At the cultivated landscape, autotrophic nitrification was controlled by soil moisture and pH, whereas heterotrophic nitrification was limited by organic C. Substrate availability was identified as the main driving variable of autotrophic nitrifiers at the pasture landscape.en_US
dc.language.isoen_USen_US
dc.titleNitrification at the community level across a cultivated and a pasture landscapeen_US
thesis.degree.departmentSoil Scienceen_US
thesis.degree.disciplineSoil Scienceen_US
thesis.degree.grantorUniversity of Saskatchewanen_US
thesis.degree.levelDoctoralen_US
thesis.degree.nameDoctor of Philosophy (Ph.D.)en_US
dc.type.materialtexten_US
dc.type.genreThesisen_US
dc.contributor.committeeMemberde Jong, Eeltjeen_US


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