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dc.contributor.advisorPaterson, Phyllis G.en_US
dc.creatorBobyn, Patricia Joanen_US
dc.date.accessioned2003-10-31T13:30:29Zen_US
dc.date.accessioned2013-01-04T05:07:29Z
dc.date.available2004-10-31T08:00:00Zen_US
dc.date.available2013-01-04T05:07:29Z
dc.date.created2003-10en_US
dc.date.issued2003-10-15en_US
dc.date.submittedOctober 2003en_US
dc.identifier.urihttp://hdl.handle.net/10388/etd-10312003-133029en_US
dc.description.abstractPrimary brain injury in stroke is followed by oxidative stress and further neural damage. Glutathione (GSH) is critical in antioxidant defense. Since cysteine is limiting in GSH synthesis, Phase 1 of this study investigated the effect of a dietary sulphur amino acid deficiency (-SAA) on neural damage in global hemispheric hypoxia-ischemia (GHHI). Rats were fed a -SAA or control diet for 6 days, and subjected to GHHI after 3 days. Histologically evaluated neural damage at 7 days post hypoxia-ischemia was greater in -SAA rats. Brain GSH concentration was decreased in -SAA rats 3 days after ischemia. A cysteine precursor, L-2-oxothiazolidine-4-carboxylic acid (OTC) administered to -SAA rats did not ameliorate neural damage. GSH is decreased by protein-energy malnutrition (PEM) in some tissues. Phase 2 investigated the effect of PEM on brain oxidative stress, neural damage and behaviour after global ischemia in adult male gerbils. In a 2x2 factorial design, gerbils were fed an adequate protein (12%; C) or low protein (2%; PEM) diet for 4 weeks, then subjected to transient ischemia (I) or sham surgery (S). After 12 hours of reperfusion, brain from half the gerbils was collected for biochemical analyses. Remaining gerbils were fed pre-surgery diets for 10 more days. To assess functional consequences of ischemia, gerbils were placed in an open field on Days 3, 7 and 10 after surgery. On Day 10, viable hippocampal CA1 neurons were counted. C-I gerbils did not habituate as readily in the open field on day 3 as C-S, but normalized by day 7. PEM-I gerbils failed to habituate by day 10, traveled greater distance than other gerbils and 7 of 12 displayed thigmotaxis, a wall-hugging preference for the outer perimeter of the open field. CA1 neuron loss in I was 61.5% of S, but unaffected by PEM. Four of 12 PEM-I gerbils had marked increases in hippocampal glia. Hippocampus protein thiols were reduced by PEM and by ischemia, consistent with oxidative stress. GSH concentration, glutathione reductase activity and thiobarbituric acid reactive substances were not significantly affected by PEM or ischemia. Findings from these two studies suggest well-nourished but not nutritionally-deficient rodents tolerate a mild brain insult. This is clinically relevant because many elderly stroke victims suffer from PEM at the time of ischemia, which may compromise recovery.en_US
dc.language.isoen_USen_US
dc.subjectoxidative stressen_US
dc.subjectglutathioneen_US
dc.subjectgerbilen_US
dc.subjectstrokeen_US
dc.subjectProtein-energy malnutritionen_US
dc.titleNutritional influence on oxidative stress in global ischemiaen_US
thesis.degree.departmentNutritionen_US
thesis.degree.disciplineNutritionen_US
thesis.degree.grantorUniversity of Saskatchewanen_US
thesis.degree.levelDoctoralen_US
thesis.degree.nameDoctor of Philosophy (Ph.D.)en_US
dc.type.materialtexten_US
dc.type.genreThesisen_US
dc.contributor.committeeMemberWhiting, Susan J.en_US
dc.contributor.committeeMemberSaucier, Deborah M.en_US
dc.contributor.committeeMemberJuurlink, Bernhard H. J.en_US
dc.contributor.committeeMemberHamilton, Donald L.en_US
dc.contributor.committeeMemberBray, Tammy M.en_US
dc.contributor.committeeMemberZello, Gordon A.en_US


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