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dc.contributor.advisorLin, Yen-Hanen_US
dc.creatorYang, Danmeien_US
dc.date.accessioned2007-12-04T11:55:58Zen_US
dc.date.accessioned2013-01-04T05:09:45Z
dc.date.available2007-12-05T08:00:00Zen_US
dc.date.available2013-01-04T05:09:45Z
dc.date.created2007-12en_US
dc.date.issued2007-12-05en_US
dc.date.submittedDecember 2007en_US
dc.identifier.urihttp://hdl.handle.net/10388/etd-12042007-115558en_US
dc.description.abstractThe global gene expression profiles of industrial strains of Saccharomyces cerevisiae responding to nitrogen deficiency and very high sugar concentrations stresses were determined by oligonucleotide microarray analysis of ~ 6200 yeast open reading frames. Genomics analysis showed that 400 genes in S. cerevisiae was differentially expressed by more than 1.5-fold compared with controls at late-logarithmic phase of fermentation, as the yeast adapted to changing nutritional, environmental and physiological conditions. The genes of many pathways are regulated in a highly coordinated manner. The repressed expression of GDH1 and up-regulation of ARO10 within the contrast of Q270/Q10 indicated high energy demanding of yeast cells under high sugar stress. Activities of G3P shuttle indicated that under very high gravity environment, sufficient assimilatory nitrogen enhances yeast’s ability of redox balancing, and therefore higher stress-tolerance and higher fermentation efficiency of yeast. Under contrast W270/Q270, the up-regulation of DUR1,2 responsible for urea degradation induces the glutamate biosynthesis and the consumption of -ketoglutarate. This may indicate that higher nitrogen level would enable higher activities in the TCA cycle, and therefore generate more energy for biosynthesis and yeast cell proliferation under very high gravity fermentation conditions. Nitrogen metabolism was also stimulated by high nitrogen level when yeast was grown in very high gravity environment.en_US
dc.language.isoen_USen_US
dc.subjectmetabolic pathwayen_US
dc.subjectSaccharomyces cerevisiaeen_US
dc.subjectgene expressionen_US
dc.subjectmicroarrayen_US
dc.titleGene expression profiling in Saccharomyces cerevisiae grown at different specific gravity environmentsen_US
thesis.degree.departmentChemical Engineeringen_US
thesis.degree.disciplineChemical Engineeringen_US
thesis.degree.grantorUniversity of Saskatchewanen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Science (M.Sc.)en_US
dc.type.materialtexten_US
dc.type.genreThesisen_US
dc.contributor.committeeMemberWu, Fang-Xiangen_US
dc.contributor.committeeMemberEvitts, Richard W.en_US
dc.contributor.committeeMemberNemati, Mehdien_US


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