Cloning, expression, and characterization of lactic acid bacteria recombinant prolidases
| dc.contributor.advisor | Tanaka, Takuji | en_US |
| dc.contributor.committeeMember | Shand, Phyllis J. | en_US |
| dc.contributor.committeeMember | Korber, Darren R. | en_US |
| dc.creator | Yang, Soo In | en_US |
| dc.date.accessioned | 2007-04-14T12:38:03Z | en_US |
| dc.date.accessioned | 2013-01-04T04:29:07Z | |
| dc.date.available | 2008-04-23T08:00:00Z | en_US |
| dc.date.available | 2013-01-04T04:29:07Z | |
| dc.date.created | 2007 | en_US |
| dc.date.issued | 2007 | en_US |
| dc.date.submitted | 2007 | en_US |
| dc.description.abstract | Lactobacillus plantarum (Lb. plantarum) NRRL B4496 and Lactococcus lactis (Lc. lactis) NRRL B1821 prolidase genes were isolated, cloned, and sequenced. The sequence-confirmed genes were subcloned into the expression systems. The recombinant prolidases from the pKK223-3 systems were purified through ammonium sulphate precipitation and anion-exchange column chromatography. Recombinant Lb. plantarum prolidase, however, demonstrated a loss of activity during the purification. The following characterization work was performed on purified recombinant Lc. lactis prolidase. The mass spectroscopic result and the molecular modelling suggested a 80 kDa homodimer with two metal cations at the catalytic centre of the prolidase. The optimum temperature was 50 ºC and showed more than 50% activities between 40 and 55 ºC. The enzyme was most stable at 30 ºC and withstood 20 min of heat-treatment up to 60 ºC, however, lost activity over 70 ºC. Circular dichroism indicated a denaturation temperature of 67 ºC. The optimum pH was 6.5 for hydrolyzing Leu-Pro and the enzyme did not display any activity below pH 5.5 nor above pH 7 with this peptide. However, Phe-Pro was hydrolyzed the fastest at pH 7 and Arg-Pro had a maximum rate at pH 9. This metallopeptidase exhibited a broad range of metal cation preference, hydrolyzing Leu-Pro with Mn++, Co++, Zn++, Ca++, and Mg++. Further kinetic analysis showed unusual allostery of the enzyme (Hill coefficient: 1.3). The unique substrate intakes onGlu-Pro and tripeptides were observed while Val-Pro was not hydrolyzed. The molecular modelling of this prolidase suggested a difference in the substrate specificity resulting from a loop structure, L33 to R40, near the substrate binding site. | en_US |
| dc.identifier.uri | http://hdl.handle.net/10388/etd-04142007-123803 | en_US |
| dc.language.iso | en_US | en_US |
| dc.subject | Lactic acid bacteria | en_US |
| dc.subject | PepQ | en_US |
| dc.subject | prolidase | en_US |
| dc.title | Cloning, expression, and characterization of lactic acid bacteria recombinant prolidases | en_US |
| dc.type.genre | Thesis | en_US |
| dc.type.material | text | en_US |
| thesis.degree.department | Applied Microbiology and Food Science | en_US |
| thesis.degree.discipline | Applied Microbiology and Food Science | en_US |
| thesis.degree.grantor | University of Saskatchewan | en_US |
| thesis.degree.level | Masters | en_US |
| thesis.degree.name | Master of Science (M.Sc.) | en_US |