Lipid remodeling during early development of rainbow trout (Oncorhynchus mykiss)
Date
2024-02-29
Authors
Wang, Chao
Gong, Yufeng
Deng, Fuchang
Liu, Xin
Ding, Enming
Zhang, Shuyi
Ma, Xiao
Raine, Jason
Jones, Paul
Tang, Song
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Elsevier
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Article
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Abstract
Lipids play a crucial role in a range of physiological processes, with a particular significance during the early development stages. However, our understanding of lipid remodeling in cold-water salmon species during these critical early developmental phases remains limited. To address this, a comprehensive lipidomic study was conducted using rainbow trout (Oncorhynchus mykiss) as a model and four critical developmental stages were selected for analysis (i.e., newly fertilized eggs, eyed stage, post-hatch sac-fry stage, and swim-up stage). As expected, our results demonstrated significant lipid alterations during early development of rainbow trout. Through fuzzy c-mean clustering analysis, our study further provided a detailed description on the dynamic changes and regulation of the lipidome. Most unsaturated phosphatidylcholines (PCs) excluding very long-chain polyunsaturated PCs and all polyunsaturated triacylglycerols (TGs) were continuously consumed, suggesting that they are the major energy sources and/or precursor substances for early-stage development in rainbow trout. Gradual increases in very long-chain polyunsaturated phosphatidylethanolamines (PEs) and PCs, phosphatidylserines (PSs), as well as saturated and monounsaturated sphingomyelins (SMs) were observed in this study, which may closely associate with retina and brain development, and the myelination process. Additionally, diacylglycerol (DG), a well-known second messenger, displayed continuous synthesis throughout embryogenesis in this study. In addition to the abovementioned lipid dynamics, our study also found distinct patterns of change in certain glycerophospholipids (GP) isomers, while all glycerolipid (GL) isomers exhibited consistent trends in alteration, indicating different GP isomers may have different biological functions or activities for early development. Overall, this study emphasized the significant roles of lipids during the early development of rainbow trout and served as an informational data set for fish development-related research.
Description
The version of record of this article, first published in Aquaculture Reports, is available online at Publisher’s website: https://doi.org/10.1007/s00267-023-01884-z
Keywords
Early development, Lipidomics, Rainbow trout, Dynamic changes, Sac-fry stage
Citation
Wang C, Gong Y, Deng F, Liu X, Ding E, Zhang S, Ma X, Raine J, Jones PD, Tang S, Giesy JP. Lipid remodeling during early development of rainbow trout (Oncorhynchus mykiss). Aquaculture Reports. 2024 Apr 1;35:102005.
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DOI
10.1016/j.aqrep.2024.102005