Contrasting Nitrogen Fertilization and Brassica napus (Canola) Variety Development Impact Recruitment of the Root-Associated Microbiome
| dc.contributor.author | Li, Yunliang | |
| dc.contributor.author | Vail, Sally L. | |
| dc.contributor.author | Arcand, Melissa M. | |
| dc.contributor.author | Helgason, Bobbi | |
| dc.date.accessioned | 2023-10-04T17:07:35Z | |
| dc.date.available | 2023-10-04T17:07:35Z | |
| dc.date.issued | 2023 | |
| dc.description | © 2023 The American Phytopathological Society | en_US |
| dc.description.abstract | Canola (Brassica napus) is an important broadacre crop, produced under high nitrogen (N) fertilizer application. Modern canola varieties are developed under high N rates but the impacts on root-associated microbiomes of different varieties are unknown. We studied eight canola varieties spanning historical Canadian spring canola development at two sites under high and low N fertility and characterized bacterial and fungal microbiomes in the root and rhizosphere using amplicon sequencing. Environmental conditions and the resulting canola varietal responses strongly affected the root-associated bacterial and fungal microbiomes. Microbes regulated by N fertility in each canola variety were mainly Gammaproteobacteria, Bacteroidia, Actinobacteria, Sordariomycetes, Dothideomycetes, and Agaricomycetes classes. Differentially abundant (DA) microbial taxa showed that N more strongly enriched bacteria in the roots and fungi in the rhizosphere. Each variety had its specific pattern of DA amplicon sequence variants (ASVs) responding to soil N availability, and the profile of DA-ASVs in paired canola varieties were also altered by soil N availability, especially bacteria in the rhizosphere. The yield was strongly associated with a subset of microbial taxa, mainly from Proteobacteria, Actinobacteriota, and Ascomycota. These variety-dependent responses to N and links to yield performance make the root-associated microbiome a promising target for improving the agronomic performance of canola by manipulating microorganisms tailored to soil fertility and plant genotype. | en_US |
| dc.description.sponsorship | Plant Phenotyping and Imaging Research Centre; the Canola Council of Canada, Alberta Canola, SaskCanola and Manitoba Canola Growers Association; and the Government of Canada under the Canadian Agricultural Partnership's AgriScience Program, a federal, provincial, territorial initiative | en_US |
| dc.description.version | Peer Reviewed | en_US |
| dc.identifier.citation | Li, Y., Vail, S. L., Arcand, M. M., & Helgason, B. L. (2023). Contrasting Nitrogen Fertilization and Brassica napus (Canola) Variety Development Impact Recruitment of the Root-Associated Microbiome. Phytobiomes Journal, 7(1), 125–137. https://doi.org/10.1094/PBIOMES-07-22-0045-R/ASSET/IMAGES/LARGE/PBIOMES-07-22-0045-RF4.JPEG | en_US |
| dc.identifier.doi | 10.1094/PBIOMES-07-22-0045-R | |
| dc.identifier.uri | https://hdl.handle.net/10388/15112 | |
| dc.language.iso | en | en_US |
| dc.publisher | APS Publications | en_US |
| dc.rights | Attribution 2.5 Canada | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/2.5/ca/ | * |
| dc.subject | Brassica napus | en_US |
| dc.subject | canola variety | en_US |
| dc.subject | nitrogen fertilization | en_US |
| dc.subject | root-associated microbiome | en_US |
| dc.subject | yield | en_US |
| dc.title | Contrasting Nitrogen Fertilization and Brassica napus (Canola) Variety Development Impact Recruitment of the Root-Associated Microbiome | en_US |
| dc.type | Article | en_US |