COMPARATIVE PROTEOMIC STUDIES OF SPROUTED LENTIL
| dc.contributor.advisor | Vandenberg, Albert | |
| dc.contributor.committeeMember | Bett, Kirstin | |
| dc.contributor.committeeMember | Martínez-Villaluenga, Cristina | |
| dc.contributor.committeeMember | Zhang , Haixia | |
| dc.contributor.committeeMember | Kutcher, Randy | |
| dc.contributor.committeeMember | Nickerson, Michael | |
| dc.creator | Dutta, Sudipta | |
| dc.date.accessioned | 2023-03-27T21:27:30Z | |
| dc.date.available | 2023-03-27T21:27:30Z | |
| dc.date.copyright | 2023 | |
| dc.date.created | 2022-12 | |
| dc.date.issued | 2023-03-27 | |
| dc.date.submitted | December 2022 | |
| dc.date.updated | 2023-03-27T21:27:30Z | |
| dc.description.abstract | A major portion of lentil nutritional composition consists of protein and protein-derived biomolecules. Seed germination improves the protein quality. It is important to identify at what stage of seed germination the type and quality of protein improvement occurs. This study aimed to elucidate the molecular mechanism of seed germination in terms of proteomic changes that occur during distinct stages of seed germination and post germination in seven lentil species. Four seed germination phases including control or dry seed as well as one post-germination phase were defined in this study with six lentil species where phases I, II, and III occur during the seed germination, and phase IV is the post-germination phase. The genotype IG 72815 had the smallest seed size among the genotypes studied and CDC redberry had the largest seed size. Imbibition period was the longest for CDC redberry, while period of seed germination was less. For genotype with smaller seed size showed the opposite phenomenon. The protease activity of all eight genotypes representing all lentil species began to increase during phase III, except for BGE 016880 in which protease activity started increasing in phase IV. For IG 72805 and IG 72815, the protease activity was the highest overall and then reduced in phase IV. The total essential and non-essential free amino acids released was the highest in phase IV. Among the genotypes studied, IG 110813, and IG 72623 had the highest amount of essential free amino acids (FEAAs) released during the germination phase. Among the FEAAs released, the amount of histidine was the highest followed by threonine. In phase IV, a significant increase was observed in theF EAAs isoleucine (ILE), lysine (LYS), leucine (LEU), valine (VAL), and threonine (THR) in germination phase IV. Carbohydrate degradation was initiated right from the beginning as many of the enzymes were present in control seeds, and some of the enzymes were absent in phase IV presumably due to degradation. According to this study, phase III, most of the enzymes required for the tricarboxylic acid (TCA) cycle were present, which indicates the TCA became fully functional in phase III. Protein metabolism and synthesis related to enzymes and other factors was initiated in germination phase II. Neosynthesis of lipid catabolism, lipid biosynthesis, amino acid biosynthesis, protein modification, and protein translocation related proteins were initiated in phase IV. Overall, this study found that germination phases III and IV were superior in terms of free amino acid release, and protease activity. Based on that observation, it could be concluded that phase III and phase IV were better in terms of digestibility and availability of as the protease activity and released free amino acids were found the highest in these phases. Based on proteome study it could be concluded that carbohydrate degradation followed by protein metabolism initiates seed germination phenomenon. | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.uri | https://hdl.handle.net/10388/14534 | |
| dc.language.iso | en | |
| dc.subject | Seed, Lentil, Proteomics, Germination, Free amino acids | |
| dc.title | COMPARATIVE PROTEOMIC STUDIES OF SPROUTED LENTIL | |
| dc.type | Thesis | |
| dc.type.material | text | |
| thesis.degree.department | Plant Sciences | |
| thesis.degree.discipline | Plant Sciences | |
| thesis.degree.grantor | University of Saskatchewan | |
| thesis.degree.level | Masters | |
| thesis.degree.name | Master of Science (M.Sc.) |
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