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EFFECT OF VARYING DIETARY VITAMIN A SUPPLEMENTATION LEVELS IN COMBINATION WITH ADH1C GENOTYPE ON INTRAMUSCULAR FAT DEPOSITION IN FINISHING BEEF STEERS

dc.contributor.advisorBuchanan, Fiona C.en_US
dc.contributor.advisorMcKinnon, John J.en_US
dc.contributor.committeeMemberHendrick, Steveen_US
dc.contributor.committeeMemberHogan, Natachaen_US
dc.creatorKrone, Kristinen_US
dc.date.accessioned2014-07-17T12:00:11Z
dc.date.available2014-07-17T12:00:11Z
dc.date.created2014-06en_US
dc.date.issued2014-07-16en_US
dc.date.submittedJune 2014en_US
dc.description.abstractPreviously, ADH1Cc.-64T>C was shown to have an association with intramuscular fat (IMF) in the longissimus thoracis (LT) muscle when vitamin A was limited in finishing rations of beef steers. The purpose of the current study was to determine the optimum vitamin A supplementation level, in combination with ADH1C genotype, to increase IMF of the LT muscle. Forty-five TT, 45 CT and 27 CC cross-bred steers, black in colour, were backgrounded on a commercial ration containing 3360 IU vitamin A/kg DM. During finishing the steers were randomly assigned to one of three vitamin A treatments at 25, 50 and 75% of the NRC recommendation of 2200 IU/kg DM. Treatments were administered via an oral bolus. Carcass quality was evaluated and a sample from the LT muscle was collected for analysis of IMF. A treatment x genotype interaction (P=0.04) was observed for IMF; TT steers on the 75% treatment had higher IMF relative to CT and CC steers on the same treatment. Intramuscular fat was also higher for TT steers on the 75% treatment in comparison to TT steers on the 25% treatment. Eighty-four percent of the steers graded Canada AAA. Western blot analysis showed that TT steers had higher (P=0.02) ADH1C levels in hepatic tissue. Previously, TT steers had increased IMF when fed limited vitamin A. In the current study the lack of variation between treatments and genotypes at the lower vitamin A treatment levels was likely due to the majority of the steers grading Canada AAA (USDA Choice). However, the western blot data supports that TT steers are expected to have higher IMF deposition, due to an increase production of ADH1C.en_US
dc.identifier.urihttp://hdl.handle.net/10388/ETD-2014-06-1580en_US
dc.language.isoengen_US
dc.subjectVitamin Aen_US
dc.subjectbeef cattleen_US
dc.subjectnutrigeneticsen_US
dc.titleEFFECT OF VARYING DIETARY VITAMIN A SUPPLEMENTATION LEVELS IN COMBINATION WITH ADH1C GENOTYPE ON INTRAMUSCULAR FAT DEPOSITION IN FINISHING BEEF STEERSen_US
dc.type.genreThesisen_US
dc.type.materialtexten_US
thesis.degree.departmentAnimal and Poultry Scienceen_US
thesis.degree.disciplineAnimal Scienceen_US
thesis.degree.grantorUniversity of Saskatchewanen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Science (M.Sc.)en_US

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