Upregulation of porcine epidemic diarrhea virus (PEDV) RNA translation by the nucleocapsid protein
| dc.contributor.author | Hao, Lin | |
| dc.contributor.author | Fragoso-Saavedra, Mario | |
| dc.contributor.author | Liu, Qiang | |
| dc.date.accessioned | 2024-12-23T02:55:09Z | |
| dc.date.available | 2024-12-23T02:55:09Z | |
| dc.date.issued | 2024-11 | |
| dc.description.abstract | The role of coronaviral nucleocapsid (N) protein in regulating viral translation remains poorly understood. Here, we showed that the N protein of porcine epidemic diarrhea virus (PEDV) enhances the translation of both virus-like genomic RNA (gRNA) and messenger RNA. Further characterization of the gRNA translation upregulation showed that the N-terminal domain (NTD) + Linker region plays a major role. The stem-loop 1 in the 5′ untranslated region (UTR) and the budged stem loop in the 3′UTR are required for viral translation upregulation by PEDV N protein. The signaling kinase Akt exists in three isoforms. We found that Akt1 enhances viral gRNA translation upregulation by the N protein dependent on its kinase activity. We further showed an interaction between Akt1 and PEDV N, that is abolished by the NTD + Linker region. This suggested that the enhancing effect of Akt1 on translation upregulation by the N protein does not require interaction between these two proteins. | |
| dc.description.sponsorship | This work was supported by Discovery Grants from the Natural Sciences and Engineering Research Council of Canada (RPN-2018-04138, RPN-2024-03752) to QL. LH is partially supported by a scholarship from Vaccinology and Immunotherapeutics Graduate program, School of Public Health, University of Saskatchewan. | |
| dc.description.version | Peer Reviewed | |
| dc.identifier.doi | https://doi.org/10.1016/j.virol.2024.110306 | |
| dc.identifier.uri | https://hdl.handle.net/10388/16359 | |
| dc.language.iso | en | |
| dc.publisher | Virology | |
| dc.rights | Attribution 2.5 Canada | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/2.5/ca/ | |
| dc.subject | Coronavirus | |
| dc.subject | Porcine epidemic diarrhea virus (PEDV) | |
| dc.subject | Nucleocapsid protein | |
| dc.subject | PEDV RNA translation | |
| dc.subject | Untranslated region | |
| dc.subject | Akt1 | |
| dc.subject | Protein-protein interaction | |
| dc.title | Upregulation of porcine epidemic diarrhea virus (PEDV) RNA translation by the nucleocapsid protein | |
| dc.type | Article |