Protocol for mpox virus inactivation in containment level 3 for safe handling of animal samples
Date
2025-06
Authors
Moni, Benedicte Mpia
Hurtado-Monzón, Arianna Mahely
Kessel, Jill Van
Kozak, Robert A.
Facciuolo, Antonio
Banerjee, Arinjay
Kumar, Anil
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ScienceDirect
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Article
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Abstract
High biocontainment is required to handle infectious mpox virus (MPXV) specimens, which can hinder research and diagnostic efforts during outbreaks. Here, we present a protocol for inactivating MPXV-contaminated animal samples using commercial lysis buffers. We describe steps for virus propagation and quantification and MPXV inactivation. We then detail procedures for validating the inactivation using a cell-based technique and qPCR. This protocol provides a framework for safely managing MPXV in lower-containment laboratories, facilitating downstream applications and improved outbreak management.
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Keywords
cell culture, microbiology, molecular biology
Citation
Moni, B. M., Hurtado-Monzón, A. M., Van Kessel, J., Kozak, R. A., Facciuolo, A., Banerjee, A., & Kumar, A. (2025). Protocol for mpox virus inactivation in containment level 3 for safe handling of animal samples. STAR Protocols, 6(2), 103770-. https://doi.org/10.1016/j.xpro.2025.103770
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DOI
https://doi.org/10.1016/j.xpro.2025.103770