Protocol for mpox virus inactivation in containment level 3 for safe handling of animal samples
| dc.contributor.author | Moni, Benedicte Mpia | |
| dc.contributor.author | Hurtado-Monzón, Arianna Mahely | |
| dc.contributor.author | Kessel, Jill Van | |
| dc.contributor.author | Kozak, Robert A. | |
| dc.contributor.author | Facciuolo, Antonio | |
| dc.contributor.author | Banerjee, Arinjay | |
| dc.contributor.author | Kumar, Anil | |
| dc.date.accessioned | 2025-05-01T01:52:51Z | |
| dc.date.available | 2025-05-01T01:52:51Z | |
| dc.date.issued | 2025-06 | |
| dc.description.abstract | High biocontainment is required to handle infectious mpox virus (MPXV) specimens, which can hinder research and diagnostic efforts during outbreaks. Here, we present a protocol for inactivating MPXV-contaminated animal samples using commercial lysis buffers. We describe steps for virus propagation and quantification and MPXV inactivation. We then detail procedures for validating the inactivation using a cell-based technique and qPCR. This protocol provides a framework for safely managing MPXV in lower-containment laboratories, facilitating downstream applications and improved outbreak management. | |
| dc.description.sponsorship | This study was supported by a Canadian Institutes of Health Research (CIHR) Team grant: mpox and zoonotic threats – ecological and zoonotic pool awarded to R.A.K., A.F., A.B., and A.K. VIDO receives operational funding from the Government of Saskatchewan through Innovation Saskatchewan and the Ministry of Agriculture and from the Canada Foundation for Innovation through the Major Science Initiatives Fund. | |
| dc.description.version | Peer Reviewed | |
| dc.identifier.citation | Moni, B. M., Hurtado-Monzón, A. M., Van Kessel, J., Kozak, R. A., Facciuolo, A., Banerjee, A., & Kumar, A. (2025). Protocol for mpox virus inactivation in containment level 3 for safe handling of animal samples. STAR Protocols, 6(2), 103770-. https://doi.org/10.1016/j.xpro.2025.103770 | |
| dc.identifier.doi | https://doi.org/10.1016/j.xpro.2025.103770 | |
| dc.identifier.uri | https://hdl.handle.net/10388/16897 | |
| dc.language.iso | en | |
| dc.publisher | ScienceDirect | |
| dc.rights | Attribution 2.5 Canada | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/2.5/ca/ | |
| dc.subject | cell culture | |
| dc.subject | microbiology | |
| dc.subject | molecular biology | |
| dc.title | Protocol for mpox virus inactivation in containment level 3 for safe handling of animal samples | |
| dc.type | Article |