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RAPD markers for Ascochyta resistance in lentil

dc.contributor.authorAndrahennadi, C.P.
dc.contributor.authorSlinkard, A.E.
dc.contributor.authorVandenberg, A.
dc.date.accessioned2018-09-11T20:34:48Z
dc.date.available2018-09-11T20:34:48Z
dc.date.issued1995-02-23
dc.description.abstractGenetic linkage of RAPDmarkers with genes for resistance to Ascochyta blight was studied in two crosses of lentil. DNA was extracted from F, plants of both crosses (ILL 5588 x Eston and Indianhead x Eston). Screening for Ascochyta was performed on F,-derived F, lines in a disease nursery with a susceptible check. Bulked segregant analysis was used to identify polymorphic RAPD bands in four bulks for 60 primers. Two polymorphic RAPD bands ( one each in primer #333 and 362 in the resistant bulk) were detected in ILL 5588 x Eston population, indicating linkage with the dominant gene, RuZI for Ascochyta resistance. Another polymorphic RAPD band (#338 in the susceptible bulk) was detected in Indianhead x Eston population, indicating linkage with the recessive gene, ra12. These marker linkages must be confirmed by studying individual plants so that they can be useful in the selection program for Ascochyta resistance in lentil.en_US
dc.description.versionNon-Peer Reviewed
dc.identifier.urihttp://hdl.handle.net/10388/10365
dc.language.isoenen_US
dc.relation.ispartofSoils and Crops Workshop
dc.rightsAttribution-NonCommercial-NoDerivs 2.5 Canada*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.5/ca/*
dc.subjectbulked segregant analysisen_US
dc.titleRAPD markers for Ascochyta resistance in lentilen_US
dc.typePresentationen_US

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