Characterization of pro-opiomelanocortin gene variants and their effect on carcass traits in beef cattle
| dc.contributor.advisor | Buchanan, Fiona | en_US |
| dc.contributor.committeeMember | Laarveld, Bernard | en_US |
| dc.contributor.committeeMember | Shand, Phyllis | en_US |
| dc.contributor.committeeMember | Schmutz, Sheila | en_US |
| dc.contributor.committeeMember | Soita, Henry | en_US |
| dc.creator | Deobald, Heather Maureen | en_US |
| dc.date.accessioned | 2009-08-18T14:38:43Z | en_US |
| dc.date.accessioned | 2013-01-04T04:53:31Z | |
| dc.date.available | 2010-09-17T08:00:00Z | en_US |
| dc.date.available | 2013-01-04T04:53:31Z | |
| dc.date.created | 2009 | en_US |
| dc.date.issued | 2009 | en_US |
| dc.date.submitted | 2009 | en_US |
| dc.description.abstract | Pro-opiomelanocortin is a prohormone that codes for many different peptides, some of which are involved in the appetite pathway. A single nucleotide polymorphism c.288C>T in pro-opiomelanocortin (POMC) was previously demonstrated to be associated with hot carcass weight (HCW) and shipping weight (Ship wt) in cattle. While developing a commercial real time PCR test for the POMC c.288C>T we identified a 12 bp deletion (POMC c.293_304delTTGGGGGCGCGG). The deletion results in the removal of four amino acids; valine, two glycine, and alanine. The deletion does not cause a frame shift. Both the POMC c.288C>T SNP and the deletion were genotyped in 386 crossbred steers, and evaluated for associations with carcass traits. The animals with one copy of the deletion had a significantly lower end-of-background rib-eye area (P=0.04) and carcass rib-eye area (P=0.03) when compared to animals without the deletion. A significant association with the POMC c.288C>T SNP was found with start of finishing weight (SOF WT); (P=0.04), HCW (P=0.02), average fat and grade fat (P=0.05), carcass rib-eye area (REA); (P=0.03) and marbling (P=0.02). These results suggest that it would be beneficial for beef producers to know both the deletion and the POMC c.288C>T SNP genotypes when making marketing and culling decisions. | en_US |
| dc.identifier.uri | http://hdl.handle.net/10388/etd-08182009-143843 | en_US |
| dc.language.iso | en_US | en_US |
| dc.subject | POMC | en_US |
| dc.subject | cattle | en_US |
| dc.title | Characterization of pro-opiomelanocortin gene variants and their effect on carcass traits in beef cattle | en_US |
| dc.type.genre | Thesis | en_US |
| dc.type.material | text | en_US |
| thesis.degree.department | Animal and Poultry Science | en_US |
| thesis.degree.discipline | Animal and Poultry Science | en_US |
| thesis.degree.grantor | University of Saskatchewan | en_US |
| thesis.degree.level | Masters | en_US |
| thesis.degree.name | Master of Science (M.Sc.) | en_US |