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Assessment of Rapid and Conventional RT-qPCR-Based Systems for Wastewater Surveillance

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dc.contributor.authorAsadi, Mohsen
dc.contributor.authorHamilton, Daniel
dc.contributor.authorShomachuk, Corwyn
dc.contributor.authorOloye, Femi F.
dc.contributor.authorDe Lange, Chantel
dc.contributor.authorLiang, Jiaqi
dc.contributor.authorXia, Pu
dc.contributor.authorOsunla, Charles A.
dc.contributor.authorCantin, Jenna
dc.contributor.authorMejia, Edgard M.
dc.contributor.authorGregorchuk, Branden S. J.
dc.contributor.authorBecker, Michael G.
dc.contributor.authorMangat, Chand
dc.contributor.authorBrinkmann, Markus
dc.contributor.authorJones, Paul D.
dc.contributor.authorGiesy, John P.
dc.contributor.authorMcPhedran, Kerry M.
dc.date.accessioned2024-10-30T07:37:37Z
dc.date.available2024-10-30T07:37:37Z
dc.date.issued2024-09-05
dc.descriptionPre-print version. Author submitted version without peer-review. Original article DOI: 10.1021/acsestwater.4c00167
dc.description.abstractConventional wastewater surveillance (WS) relies on highly trained personnel, advanced instrumentation, and significant resources, making the development and use of simple, rapid, and sensitive alternative technologies valuable for reducing costs, time, and labor intensity. For the first time, this study investigated the use of two well-developed rapid systems, including the GeneXpert and LuminUltra, in parallel with a conventional WS reference methodology for the assessment of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in three cities: Saskatoon, Prince Albert, and North Battleford, Saskatchewan, Canada. RNA extractions from wastewater samples were carried out for the conventional reference and LuminUltra methods, while GeneXpert was used for both raw and concentrated wastewater samples. Bland–Altman plots showed a combination of systematic bias and random error between these real-time reverse transcription-quantitative polymerase chain reaction (RT-qPCR)-based systems. Additionally, results indicated the reasonable performance of GeneXpert in viral detection with a sensitivity rate of >98%, as compared to the conventional reference methodology of 100% and LuminUltra with >65%. A Spearman correlation test showed meaningful relationships between the GeneXpert and conventional reference methodology viral level results across all cities, indicating GeneXpert’s reliability for accurate viral detection and disease prevalence determination, specifically in limited-resource communities, with a shorter processing time and cost-effectiveness in analysis.
dc.description.sponsorshipGlobal Water Futures program, Canada First Research Excellence Fund #419205 Discovery Grant from the Natural Sciences and Engineering Research Council of Canada (Project # 326415-07)
dc.description.versionNon-Peer Reviewed
dc.identifier.citationMohsen Asadi, Daniel Hamilton, Corwyn Shomachuk, Femi F. Oloye, Chantel De Lange, Jiaqi Liang, Pu Xia, Charles A. Osunla, Jenna Cantin, Edgard M. Mejia, Branden S. J. Gregorchuk, Michael G. Becker, Chand Mangat, Markus Brinkmann, Paul D. Jones, John P. Giesy, and Kerry N. McPhedran ACS ES&T Water 2024 4 (10), 4333-4342 DOI: 10.1021/acsestwater.4c00167
dc.identifier.doi10.1021/acsestwater.4c00167
dc.identifier.urihttps://hdl.handle.net/10388/16226
dc.language.isoen
dc.publisherACS Publications
dc.subjectwastewater surveillance
dc.subjectviral disease prevalence
dc.subjectconventional reference methodology
dc.subjectGeneXpert
dc.subjectLuminUltra
dc.titleAssessment of Rapid and Conventional RT-qPCR-Based Systems for Wastewater Surveillance
dc.typePreprint

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