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Immunology of BCG vaccination in mice : implications for tuberculosis vaccination and for the use of BCG as a recombinant vaccine vector

Date

2000-01-01

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Doctoral

Abstract

The effect of vaccination dose on the immune response of mice to Mycobacterium bovis Bacille Calmette-Guerin (BCG) was examined. To assess T helper cell-type 1 (Th1) and -type 2 (Th2) responses, a very sensitive ELISPOT assay was developed. Using this assay, the numbers of BCG-specific interferon ɣ- and interleukin 4-producing spleen cells were used as indicators of the Th1 and Th2 responses, respectively. IgG1 and IgG2a serum antibody titers were also assessed. Mice were immunized with different doses of BCG, and their immune response to BCG antigen was determined at various times after vaccination. Low dose immunized mice developed predominantly Th1 responses and little antibody, while those given higher numbers of bacilli generated a mixed Th1/Th2 response and had significant antibody titers. Furthermore, mice given the lower doses of BCG developed an immune imprint that enabled them to resist development of a Th2 response on subsequent challenge with a higher dose of BCG, which generates a Th2 response in previously unexposed mice. The immune response of mice immunized with recombinant BCG (rBCG) expressing 'Escherichia coli' β-galactosidase was also characterized. Th1 cells predominated in the immune response to BCG antigen as well as to the expressed β-galactosidase antigen in low dose vaccinated mice. Moreover, the Th1 response to both antigens was stable after high dose challenge of low dose vaccinated animals indicating that immune imprinting had been established both to BCG and to the recombinant β-galactosidase antigen. The results indicate that the immune state that develops in mice upon vaccination with BCG depends on the initial dose of antigen administered, with low amounts of antigen leading to Th1 immunity, and relatively high amounts of antigen leading to Th2 immune responses. 'Mycobacterium tuberculosis ' is only controlled effectively by a cell-mediated immune response. Immunization with a relatively low dose of BCG, rather than the current standard dose, may provide greater immune protection against 'M. tuberculosis'. Moreover, low dose vaccination with rBCG expressing proteins of leishmania or HIV promises to be an effective means of vaccination against diseases caused by these pathogens, which can only be contained by a Th1 response.

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Degree

Doctor of Philosophy (Ph.D.)

Department

Microbiology and Immunology

Program

Microbiology and Immunology

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