ERYTHROCYTE DEFORMABILITY AS A BIOCHEMICAL INDICATOR OF ZINC STATUS
| dc.contributor.advisor | Paterson, Phyllis | |
| dc.contributor.committeeMember | Card, R.T. | |
| dc.contributor.committeeMember | Christensen, D. A. | |
| dc.contributor.committeeMember | Hawes, E.M. | |
| dc.contributor.committeeMember | Foldvari, M. | |
| dc.creator | Robinson, Lynne J. | |
| dc.date.accessioned | 2023-11-17T20:57:47Z | |
| dc.date.available | 2023-11-17T20:57:47Z | |
| dc.date.issued | 1997 | |
| dc.date.submitted | 1997 | en_US |
| dc.description.abstract | It was hypothesized that zinc deficiency exerts an effect on RBC deformability through its influence on the plasma membrane and that this would form the basis for a biochemical indicator of zinc status. Weanling, male Sprague-Dawley rats were fed ad libitum modified AIN-93G diets containing 3 mg zinc/kg diet (-Zn; n=10) for six weeks. Control rats were pair-fed (+ZnPF; n=10) or fed ad libitum (+ZnAL; n=9) diets with 50 mg zinc/kg diet. The mean (±SEM) weight gain (g) for the three groups was - Zn=161.6 ± 8.7, +ZnPF=203.0 ± 12.8 and +ZnAL=346.5 ± 11.5. Zinc deficiency and depressed food intake significantly decreased plasma and tibia zinc concentration (P<0.05). Experimental treatments had small but statistically significant effects on some parts of the hematological profile. RBC deformability was measured on whole blood as a function of shear stress in the ektacytometer. Elongation index (EI), the ratio of length to width of the diffraction pattern of deforming cells, was. measured. As analyzed by one-factor ANOVA, maximum elongation index (Elmax), a measure of average deformability of the cell population, was not altered by zinc deficiency. The mean (±SEM) values of Elmax for the three groups were -Zn=0.55 ± 0.01, +ZnPF=0.56 ± 0.01 and +ZnAL=0.56±0.01. EI was plotted against shear stress and the initial slope of the curve, a measure of membrane deformability, was not changed by zinc deficiency. The mean (±SEM) values of slope for the three groups were -Zn=0.023± 0.002, +ZnPF =0.021 ± 0.001 and +ZnAL=0.051± 0.027. No effect of zinc deficiency was found for deformability of RBC suspensions heated at 48°C for 6 minutes. The results of this study suggest that RBC deformability can not be used as a functional indicator of zinc status. | en_US |
| dc.identifier.uri | https://hdl.handle.net/10388/15277 | |
| dc.title | ERYTHROCYTE DEFORMABILITY AS A BIOCHEMICAL INDICATOR OF ZINC STATUS | en_US |
| dc.type.genre | Thesis | en_US |
| thesis.degree.department | Nutrition and Dietetics | en_US |
| thesis.degree.grantor | University of Saskatchewan | en_US |
| thesis.degree.level | Masters | en_US |
| thesis.degree.name | Master of Science (M.Sc.) | en_US |