Unveiling the Brazilian kefir microbiome: Discovery of a novel Lactobacillus kefiranofaciens (LkefirU) genome and in silico prospection of bioactive peptides with potential anti-Alzheimer properties
Date
2024-09-20
Authors
Silva, Matheus H.
Batista, Letícia L.
Malta, Serena M.
Santos, Ana C. C.
Mendes-Silva, Ana Paula
Bonetti, Ana M.
Ueira-Vieira, Carlos
dos Santos, Anderson R.
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BMC Genomics
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Article
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Abstract
Background
Kefir is a complex microbial community that plays a critical role in the fermentation and production of bioactive peptides, and has health-improving properties. The composition of kefir can vary by geographic localization and weather, and this paper focuses on a Brazilian sample and continues previous work that has successful anti-Alzheimer properties. In this study, we employed shotgun metagenomics and peptidomics approaches to characterize Brazilian kefir further.
Results
We successfully assembled the novel genome of Lactobacillus kefiranofaciens (LkefirU) and conducted a comprehensive pangenome analysis to compare it with other strains. Furthermore, we performed a peptidome analysis, revealing the presence of bioactive peptides encrypted by L. kefiranofaciens in the Brazilian kefir sample, and utilized in silico prospecting and molecular docking techniques to identify potential anti-Alzheimer peptides, targeting β-amyloid (fibril and plaque), BACE, and acetylcholinesterase. Through this analysis, we identified two peptides that show promise as compounds with anti-Alzheimer properties.
Conclusions
These findings not only provide insights into the genome of L. kefiranofaciens but also serve as a promising prototype for the development of novel anti-Alzheimer compounds derived from Brazilian kefir.
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Keywords
Lactobacillus kefiranofaciens, Metagenome, Kefir, Next Generation DNA Sequencing, Peptidome, Alzheimer’s disease
Citation
Silva, M.H., Batista, L.L., Malta, S.M. et al. Unveiling the Brazilian kefir microbiome: discovery of a novel Lactobacillus kefiranofaciens (LkefirU) genome and in silico prospection of bioactive peptides with potential anti-Alzheimer properties. BMC Genomics 25, 884 (2024). https://doi.org/10.1186/s12864-024-10695-3
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DOI
https://doi.org/10.1186/s12864-024-10695-3