Unveiling the Brazilian kefir microbiome: Discovery of a novel Lactobacillus kefiranofaciens (LkefirU) genome and in silico prospection of bioactive peptides with potential anti-Alzheimer properties
| dc.contributor.author | Silva, Matheus H. | |
| dc.contributor.author | Batista, Letícia L. | |
| dc.contributor.author | Malta, Serena M. | |
| dc.contributor.author | Santos, Ana C. C. | |
| dc.contributor.author | Mendes-Silva, Ana Paula | |
| dc.contributor.author | Bonetti, Ana M. | |
| dc.contributor.author | Ueira-Vieira, Carlos | |
| dc.contributor.author | dos Santos, Anderson R. | |
| dc.date.accessioned | 2024-10-17T02:47:02Z | |
| dc.date.available | 2024-10-17T02:47:02Z | |
| dc.date.issued | 2024-09-20 | |
| dc.description.abstract | Background Kefir is a complex microbial community that plays a critical role in the fermentation and production of bioactive peptides, and has health-improving properties. The composition of kefir can vary by geographic localization and weather, and this paper focuses on a Brazilian sample and continues previous work that has successful anti-Alzheimer properties. In this study, we employed shotgun metagenomics and peptidomics approaches to characterize Brazilian kefir further. Results We successfully assembled the novel genome of Lactobacillus kefiranofaciens (LkefirU) and conducted a comprehensive pangenome analysis to compare it with other strains. Furthermore, we performed a peptidome analysis, revealing the presence of bioactive peptides encrypted by L. kefiranofaciens in the Brazilian kefir sample, and utilized in silico prospecting and molecular docking techniques to identify potential anti-Alzheimer peptides, targeting β-amyloid (fibril and plaque), BACE, and acetylcholinesterase. Through this analysis, we identified two peptides that show promise as compounds with anti-Alzheimer properties. Conclusions These findings not only provide insights into the genome of L. kefiranofaciens but also serve as a promising prototype for the development of novel anti-Alzheimer compounds derived from Brazilian kefir. | |
| dc.description.sponsorship | Federal University of Uberlandia and the Institute of Biotechnology Fundação de Amparo à Pesquisa de Minas Gerais Conselho Nacional de Desenvolvimento Científico e Tecnológico Coordenação de Aperfeiçoamento de Pessoal de Ensino Superior Financiadora de Estudos e Projetos | |
| dc.description.version | Peer Reviewed | |
| dc.identifier.citation | Silva, M.H., Batista, L.L., Malta, S.M. et al. Unveiling the Brazilian kefir microbiome: discovery of a novel Lactobacillus kefiranofaciens (LkefirU) genome and in silico prospection of bioactive peptides with potential anti-Alzheimer properties. BMC Genomics 25, 884 (2024). https://doi.org/10.1186/s12864-024-10695-3 | |
| dc.identifier.doi | https://doi.org/10.1186/s12864-024-10695-3 | |
| dc.identifier.uri | https://hdl.handle.net/10388/16184 | |
| dc.language.iso | en | |
| dc.publisher | BMC Genomics | |
| dc.rights | Attribution-NonCommercial 2.5 Canada | en |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc/2.5/ca/ | |
| dc.subject | Lactobacillus kefiranofaciens | |
| dc.subject | Metagenome | |
| dc.subject | Kefir | |
| dc.subject | Next Generation DNA Sequencing | |
| dc.subject | Peptidome | |
| dc.subject | Alzheimer’s disease | |
| dc.title | Unveiling the Brazilian kefir microbiome: Discovery of a novel Lactobacillus kefiranofaciens (LkefirU) genome and in silico prospection of bioactive peptides with potential anti-Alzheimer properties | |
| dc.type | Article |