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Saskatchewan inoculants for chickpea and bean

Date

1996-02-22

Authors

Walley, F.L.
van Kessel, C.
Hnatowich, G.L.

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Abstract

A collaborative research study between the University of Saskatchewan, the Saskatchewan Wheat Pool, MicroBio Rhizogen Corp. and Westco Fertilizers Ltd. was initiated to identify and develop effective rhizobial inoculants for chickpea and bean grown in Saskatchewan. A large collection of chickpea and bean Rhizobium strains were procured from laboratories around the world. Using a series of growth chamber experiments, the Rhizobium strains were ranked in accordance to their ability to promote growth and enhance N accumulation. Although some Rhizobium strains were equally effective at promoting growth of both desi- and kabuli-type chickpea, the ability of some strains to promote growth depended on the host chickpea variety. Following initial screening, six chickpea Rhizobium strains (BCF6, BCF7, BCF11, BCF14, BCF31 and BCF32) and four bean Rhizobium strains (MWB6, MWB17, MWB3 1 and MWB39) were identified as potentially superior strains and were further evaluated in the first year of field studies, conducted at three sites in 1995. Field data confirmed that the different Rhizobium strains differed in their ability to enhance crop growth and seed yield of chickpea and bean. Biological nitrogen fixation was estimated using the N difference method and the l5N natural abundance technique. Both techniques provided similar estimates of N2 fixation. Preliminary estimates of N2 fixation (15N natural abundance technique) from a single site indicate that both desi- and kabuli-type chickpea responded favorably to inoculation with Rhizobium strain BCF32. As much as 94% of the N accumulated by desi-type chickpea and 81% of the N accumulated by kabuli-type chickpea was derived via N2 fixation.

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Soils and Crops Workshop

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