Immunomodulating effect of (1→3, 1→4) β-glucan, derived from oats, in mice infected with eimeria vermiformis
| dc.contributor.committeeMember | Laarveld, Bernard | en_US |
| dc.creator | Yun, Cheol Heui | en_US |
| dc.date.accessioned | 2004-10-21T00:00:48Z | en_US |
| dc.date.accessioned | 2013-01-04T05:02:28Z | |
| dc.date.available | 1997-01-01T08:00:00Z | en_US |
| dc.date.available | 2013-01-04T05:02:28Z | |
| dc.date.created | 1997-01 | en_US |
| dc.date.issued | 1997-01-01 | en_US |
| dc.date.submitted | January 1997 | en_US |
| dc.description.abstract | Coccidiosis, caused mainly by Eimeria spp., is controlled primarily by chemotherapy. Emergence of drug resistance increases the need to develop vaccines and immunostimulants to control this disease. Beta-glucan (1→3, 1→6) from yeast and fungi non-specifically enhances host resistance to bacterial, viral, protozoan and fungal infections. β-glucan (1→3, 1→4) is present in oats and its immunostimulating properties are unclear. In experiment I oat β-glucan, in a dose and time dependent manner, stimulated release of IL-1 and, to a much lesser degree, TNF-α from murine macrophages in vitro and induced release of IL-2, IL4 and IFN-γ from cultured murine primary spleen cells. Injection of oat glucan (500 μg/0.2 ml) ip in CD-1 mice increased (P<0.01) the number of macrophages in peritoneal fluid. Survival of CD-1 mice challenged with Staphylococcus aureus was improved (P<0.05) by oat β-glucan ip 3 days pre-challenge. In experiment II oat glucan treatment sc (500 μg/0.1 ml) or ig (3 mg/0.3 ml) daily for 10 days in C57BL/6 mice immunosuppressed with dexamethasone (100 μg/d) and infected with E. vermiformis reduced fecal oocyst excretion by 23 and 34%, respectively. Oat β-glucan ip or ig reduced (P<0.001) mortality in infected, immunosuppressed mice. Oat β-glucan treatments generally increased total antibody concentrations, and anti-sporozoite and anti-merozoite IgG (P<0.05). In experiment III a single dose of oat β-glucan sc given on days -2, 0 or 2, but not 6, relative to infection with E. vermiformis on day 0, reduced (P<0.05) oocyst shedding in C57BL/6 mice. Proliferative responses of spleen cells to sporozoite antigen were increased (P<0.05) in mice treated on days -2 and 0. Oat β-glucan increased (P<0.01) the percentage of CD4⁺ T cells in MLN on day 12, and reduced the percentage of CD8⁺ cells in spleen on day 19. In conclusion, oat β-glucan showed immunostimulatory properties through enhancement of non-specific and specific humoral and cellular immune responses. Oat β-glucan treatment enhanced resistance to E. vermiformis infection in mice. These findings suggest that oat β-glucan has potential for use in livestock as an immunostimulant and for the prevention and treatment of coccidiosis. | en_US |
| dc.identifier.uri | http://hdl.handle.net/10388/etd-10212004-000048 | en_US |
| dc.language.iso | en_US | en_US |
| dc.title | Immunomodulating effect of (1→3, 1→4) β-glucan, derived from oats, in mice infected with eimeria vermiformis | en_US |
| dc.type.genre | Thesis | en_US |
| dc.type.material | text | en_US |
| thesis.degree.department | Animal and Poultry Science | en_US |
| thesis.degree.discipline | Animal and Poultry Science | en_US |
| thesis.degree.grantor | University of Saskatchewan | en_US |
| thesis.degree.level | Doctoral | en_US |
| thesis.degree.name | Doctor of Philosophy (Ph.D.) | en_US |