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Mechanism and treatment of acidosis in neonatal diarrhea

Date

2004

Journal Title

Journal ISSN

Volume Title

Publisher

ORCID

Type

Degree Level

Doctoral

Abstract

The D-isomer of lactate contributes to metabolic acidosis in diarrheic calves, and is hypothesized to originate from microbial fermentation in the gastrointestinal tract. This study aimed to identify whether D-lactate is produced in the rumen, the colon, or both and to quantify urinary excretion of D-lactate. Further objectives were to determine whether Lactobacillus GG (LGG) is a suitable treatment of D-lactic acidosis in calves and to investigate the prevalence of D-lactic acidosis in monogastrics with diarrhea. High performance liquid chromatographic methods were validated for the separation of D- and L-lactate, DL-Iactate, pyruvate and acetate in calf feces, rumen fluid and urine. Fecal, rumen, serum and urine samples were then obtained from 15 diarrheic and 10 healthy calves and analysed for D- and L-lactate, pyruvate and acetate. Neither pyruvate, acetate or L-lactate contributed significantly to acidemia in any case. L-lactate was significantly higher in the rumen contents and feces of diarrheic calves compared to healthy controls but not different in serum or urine. Rumen, fecal, serum and urine D- lactate concentrations were all significantly higher (p

Description

Keywords

Citation

Degree

Doctor of Philosophy (Ph.D.)

Department

College of Pharmacy and Nutrition

Program

College of Pharmacy and Nutrition

Part Of

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DOI

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