Mechanism and treatment of acidosis in neonatal diarrhea
Date
2004
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
ORCID
Type
Degree Level
Doctoral
Abstract
The D-isomer of lactate contributes to metabolic acidosis in diarrheic calves, and is hypothesized to originate from microbial fermentation in the gastrointestinal tract. This study aimed to identify whether D-lactate is
produced in the rumen, the colon, or both and to quantify urinary excretion of D-lactate. Further objectives were to determine whether Lactobacillus GG (LGG) is a suitable treatment of D-lactic acidosis in calves and to investigate the prevalence of D-lactic acidosis in monogastrics with diarrhea.
High performance liquid chromatographic methods were validated for the separation of D- and L-lactate, DL-Iactate, pyruvate and acetate in calf feces, rumen fluid and urine. Fecal, rumen, serum and urine samples were then obtained from 15 diarrheic and 10 healthy calves and analysed for D- and L-lactate, pyruvate and acetate. Neither pyruvate, acetate or L-lactate
contributed significantly to acidemia in any case. L-lactate was significantly higher in the rumen contents and feces of diarrheic calves compared to healthy controls but not different in serum or urine. Rumen, fecal, serum and urine D-
lactate concentrations were all significantly higher (p
Description
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Citation
Degree
Doctor of Philosophy (Ph.D.)
Department
College of Pharmacy and Nutrition
Program
College of Pharmacy and Nutrition