HPLC, NMR Based Characterization, Antioxidant and Anticancer Activities of Chemical Constituents from Therapeutically Active Fungal Endophytes
Date
2024-01-01
Authors
Shah, Waqas Hussain
Khan, Wajiha
nisa, sobia
Barfuss, Michael
Schinnerl, Johann
Bacher, Markus
Vetschera, Karin
Ali, Ashraf
Nafidi, Hiba-Allah
BIN JARDAN, YOUSEF
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The Korean Society for Microbiology and Biotechnology
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Article
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Abstract
Fungi generate different metabolites some of which are intrinsically bioactive and could therefore serve as templates for drug development. In the current study, six endophytic fungi namely Aspergillus flavus, Aspergillus tubigenesis, Aspergillus oryzae, Penicillium oxalicum, Aspergillus niger, and Aspergillus brasiliensis were isolated and identified from the medicinal plant, Silybum marianum. These endophytic fungi were identified through intra transcribed sequence (ITS) gene sequencing. The bioactive potentials of fungal extracts were investigated using several bioassays such as antibacterial activity by well-diffusion, MIC, MBC, anti-biofilm, antioxidant, and haemolysis. The Pseudomonas aeruginosa PAO1 was used to determine the antibiofilm activity. The ethyl acetate extract of Aspergillus flavus showed strong to moderate efficacy against Staphylococcus aureus, Escherichia coli, P. aeruginosa, and Bacillus spizizenii. Aspergillus flavus and Aspergillus brasiliensis exhibited significant antibiofilm activity with IC50 at 4.02 and 3.63 mg/ml, while A. flavus exhibited maximum antioxidant activity of 50.8%. Based on HPLC, LC-MS, and NMR experiments kojic acid (1) and carbamic acid (methylene-4, 1-phenylene) bis-dimethyl ester (2) were identified from A. flavus. Kojic acid exhibited DPPH free radical scavenging activity with an IC50 value of 99.3 μg/ml and moderate activity against ovarian teratocarcinoma (CH1), colon carcinoma (SW480), and non-small cell lung cancer (A549) cell lines. These findings suggest that endophytic fungi are able to produce promising bioactive compounds which deserve further investigation.2
Description
The version of record of this article, first published in the Journal of Microbiology and Biotechnology, is available online at Publisher’s website: https://doi.org/10.4014/jmb.2403.03036
Keywords
Endophytic fungi, bioactive compounds, Silybum marianum, Aspergillus, antibacterial activity, antibiofilm activity
Citation
Hashem, Amr H., et al. "Bioactive compounds and biomedical applications of endophytic fungi: a recent review." Microbial cell factories 22.1 (2023): 107.
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DOI
10.4014/jmb.2403.03036