Phosphorus fertilizer responsive bacteria and fungi in canola (Brassica napus L.) roots are correlated with plant performance
Date
2025-02-17
Authors
Liu, Mengying
Mooleki, S. Patrick
Li, Yunliang
Schneider, Dave
Kochian, Leon V
Helgason, Bobbi
Journal Title
Journal ISSN
Volume Title
Publisher
Plant and Soil
ORCID
Type
Article
Degree Level
Abstract
Background
Canola (Brassica napus L.) has high phosphorus demand, but its seedlings are sensitive to seed-placed phosphorus fertilizers. Optimizing phosphorus fertilizer management for canola is critical and can benefit from insights into the root-associated microbiota, which enhances phosphorus availability through mineralization and solubilization.
Methods
We conducted a two-year field experiment applying monoammonium phosphate fertilizer at three rates (no addition, recommended rate, and high rate at 0, 17, and 32 kg P ha−1 year−1) using two opener placements (narrow at 2.5 cm vs. wide at 10 cm). Canola performance was evaluated, and rhizosphere and root bacterial and fungal microbiota was profiled by DNA amplicon sequencing.
Results
High-rate and near-seed placement of phosphorus (32 kg P ha−1 in the 2.5 cm opener) reduced canola seedling emergence but not biomass or yield, which were higher in 2020 than in 2019. Yearly variations and plant growth stages impacted the rhizosphere and root microbiota, while phosphorus fertilization only affected the root microbiota. Phosphorus fertilization enriched Burkholderia-Caballeronia-Paraburkholderia, Luteibacter, Amaurodon, Trichoderma, and Penicillium in roots, Conversely, Chryseobacterium, Chitinophaga, Flavobacterium and Olpidium were more prevalent in roots without phosphorus addition. Yield positively correlated with the relative abundance of Burkholderia-Caballeronia-Paraburkholderia and Trichoderma in roots.
Conclusions
Phosphate fertilizer rates and placements affected canola germination but not yield. Profiling of phosphorus-responsive root microbes suggests that phosphate fertilizer rate and placement at seeding can have a lasting impact on the canola root microbiota as the plant matures, modulating plant growth responses to soil phosphorus availability.
Description
This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/s11104-025-07286-w
Liu, M., Mooleki, S.P., Li, Y., Schneider, D., Kochian, L. V., & Helgason, B. L. (2025). Phosphorus fertilizer responsive bacteria and fungi in canola (Brassica napus L.) roots are correlated with plant performance. Plant Soil. https://doi.org/10.1007/s11104-025-07286-w
Keywords
Phosphorus fertilizer management, Canola growth response, Rhizosphere and root microbiota, Phosphorus responsive bacteria and fungi
Citation
Degree
Department
Program
Advisor
Committee
Part Of
item.page.relation.ispartofseries
DOI
https://doi.org/10.1007/s11104-025-07286-w