GENETICS AND LINKAGE OF ISOZYME MARKERS AND RESISTANCE TO SEEDBORNE ASCOCHYTA INFECTION IN LENTIL
| dc.contributor.advisor | Slinkard, A. E. | |
| dc.contributor.committeeMember | Harvey, B. L. | |
| dc.contributor.committeeMember | Scoles, G. | |
| dc.contributor.committeeMember | Rowland, G. | |
| dc.contributor.committeeMember | Gossen, B. D. | |
| dc.creator | Andrahennadi, Chandra P | |
| dc.date.accessioned | 2023-05-09T19:27:43Z | |
| dc.date.available | 2023-05-09T19:27:43Z | |
| dc.date.issued | 1994-03 | |
| dc.date.submitted | March 1994 | en_US |
| dc.description.abstract | The possible linkage of isozyme and morphological trait loci with genes for resistance to ascochyta blight, caused by Ascochyta fabae f. sp. lentis Gossen et aI.,was investigated in lentil (Lens culinaris Medikus). Parent lines, F2 populations and a susceptible check, Spanish Brown lentil, were screened for ascochyta reaction in a field experiment at the North Seed Farm in Saskatoon durings ummer 1992. Parent lines were evaluated for both foliar infection, using a disease scale of 1 to 9, and percent seedborne ascochyta infection, using a seed plating technique. Individual plants in eight F2 populations were scored for percent seedborne ascochyta infection. The genetics and inheritance of 11 isozyme loci and 7 morphological trait loci were also studied in 12 F2 populations of lentil. The susceptible check, Spanish Brown lentil, was very susceptible to both foliar infection (9) and seedborne ascochyta infection (40%). Du Puy, Eston, and CDC Richlea lentils showed highly susceptible reactions to both foliar ascochyta infection (7to 9) and seedborne ascochyta infection (51to 57%). Laird lentil was moderately resistant to foliar infection (5), but susceptible to seedborne ascochyta infection (37%). Lentil lines, PI 339283, ILL 5588, PR 86-360, and PI 374118were highly resistant to both foliar ascochyta infection rating (l to 3) and seedborne ascochyta infection (0to 6.6%). The foliar ascochyta rating of Indianhead lentil was resistant (1) and moderately resistant to seedborne ascochyta infection (12%). Resistance to ascochyta in ILL 5588 lentil was due to a monogenic dominant gene (Rail). One recessive gene, ral, was responsible for resistance to ascochyta in Indianhead lentil. The F2 population of Indianhead x PI 345635 indicated that Indianhead carried two recessive duplicate genes for resistance to seedborne ascochyta infection. The F2 of Lairdx PI 374118 lentil segregated for two recessive genes for resistance to seedborne ascochyta infection ,one each from Laird and PI 374118 lentil. The inheritance of ascochyta resistance in PI 339283 lentil was governed by two dominant complementary genes. Four linkage groups of lentil, involving seven isozyme loci and yellow cotyledon color, were constructed. Pgd-p and Skdh-p were linked (35 cM), Aat-m and Me-2 were linked (4cM), Aat-p and Aco-I were linked (16 cM), and Pgm-p and yc were linked (5 cM). The dominant gene for ascochyta resistance, Ral was linked with the isozyme locus Aat-p (29 cM) and the recessive gene, ral., was linked with the isozyme locus Pgd-p (28 cM). Even though, both isozyme linkages are weak, they can be used to study these areas of the chromosomes to help screen for markers more closely linked with genes for resistance to seedborne ascochyta infection. | en_US |
| dc.identifier.uri | https://hdl.handle.net/10388/14663 | |
| dc.title | GENETICS AND LINKAGE OF ISOZYME MARKERS AND RESISTANCE TO SEEDBORNE ASCOCHYTA INFECTION IN LENTIL | en_US |
| dc.type.genre | Thesis | en_US |
| thesis.degree.department | Plant Sciences | en_US |
| thesis.degree.discipline | Crop Science and Plant Ecology | en_US |
| thesis.degree.grantor | University of Saskatchewan | en_US |
| thesis.degree.level | Masters | en_US |
| thesis.degree.name | Bachelor of Arts and Science (B.A.&Sc.) | en_US |