Repository logo
HARVEST
 

Putative mixotrophic nitrifying-denitrifying Gammaproteobacteria implicated in nitrogen cycling within the ammonia/oxygen transition zone of an oil sands pit Lake

Simple item page
dc.contributor.authorMori, Jiro F.
dc.contributor.authorChen, Lin-Xing
dc.contributor.authorJessen, Gerdhard L.
dc.contributor.authorRudderham, Sarah B.
dc.contributor.authorMcBeth, Joyce M.
dc.contributor.authorLindsay, Matthew B. J.
dc.contributor.authorSlater, Gregory F.
dc.contributor.authorBanfield, Jillian F.
dc.contributor.authorWarren, Lesley A.
dc.date.accessioned2019-11-07T16:12:11Z
dc.date.available2019-11-07T16:12:11Z
dc.date.issued2019-10-24
dc.description.abstractAnthropogenically-impacted environments offer the opportunity to discover novel microbial species and metabolisms, which may be undetectable in natural systems. Here, a combined metagenomic and geochemical study in Base Mine Lake, Alberta, Canada, which is the only oil sands end pit lake to date, revealed that nitrification was performed by members from Nitrosomonadaceae, Chloroflexi and unclassified Gammaproteobacteria “MBAE14.” While Nitrosomonadaceae and Chloroflexi groups were relatively abundant in the upper oxygenated zones, MBAE14 dominated the hypoxic hypolimnetic zones (approximately 30% of total microbial communities); MBAE14 was not detected in the underlying anoxic tailings. Replication rate analyses indicate that MBAE14 grew in metalimnetic and hypolimnetic water cap regions, most actively at the metalimnetic, ammonia/oxygen transition zone consistent with it putatively conducting nitrification. Detailed genomic analyses of MBAE14 evidenced both ammonia oxidation and denitrification into dinitrogen capabilities. However, the absence of known CO2-fixation genes suggests a heterotrophic denitrifying metabolism. Functional marker genes of ammonia oxidation (amo and hao) in the MBAE14 genome are homologous with those conserved in autotrophic nitrifiers, but not with those of known heterotrophic nitrifiers. We propose that this novel MBAE14 inhabits the specific ammonia-rich, oxygen and labile organic matter-limited conditions occurring in Base Mine Lake which selectively favors mixotrophic coupled nitrifier denitrification metabolism. Our results highlight the opportunities to better constrain biogeochemical cycles from the application of metagenomics to engineered systems associated with extractive resource sectors.en_US
dc.description.sponsorshipSyncrude Canada Ltd.en_US
dc.identifier.citationMori, J.F., Chen, L.-X., Jessen, G.L., Rudderham, S.B., McBeth, J.M., Lindsay, M.B.J., Slater, G.F., Banfield, J.F., & Warren, L.A. (2019) Putative mixotrophic nitrifying-denitrifying Gammaproteobacteria implicated in nitrogen cycling within the ammonia/oxygen transition zone of an oil sands pit lake. Frontiers in Microbiology, 10, 2435 (1–15). https:doi.org/10.3389/fmicb.2019.02435en_US
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/10388/12443
dc.language.isoenen_US
dc.publisherFrontiersen_US
dc.subjectoil sandsen_US
dc.subjectend pit lakeen_US
dc.subjectgeochemistryen_US
dc.subjectmicrobiologyen_US
dc.subjectmine wasteen_US
dc.subjectnitrogen cyclingen_US
dc.titlePutative mixotrophic nitrifying-denitrifying Gammaproteobacteria implicated in nitrogen cycling within the ammonia/oxygen transition zone of an oil sands pit Lakeen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
[30] Mori et al. (2019) Frontiers Microbiol.pdf
Size:
3.35 MB
Format:
Adobe Portable Document Format
Description:
Main Article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.28 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Geological Sciences